A straightforward method to conjugate antibodies to oligonucleotides – preparation, purification and their applications
A comparison of Dynamic Biosensors’ antibody-oligonucleotide conjugation kit with similar commercially available kits demonstrates significantly better purity, with less excess free DNA compared to competitor kits (A) and higher yields of the desired conjugates (B).
Figure I Comparison chromatograms of two different methods of preparing and purifying conjugates. A Used protocol from a competitor. B Protocol described here with subsequent proFIRE® purification.
PURE PROTEIN-DNA CONJUGATES
Well-defined protein-DNA and antibody-DNA conjugates are a very important tool for many bioanalytical applications including immunoassays, imaging techniques, protein characterization technologies and surface bio-functionalization.
Yet, preparing pure bioconjugates can still be a challenging process.
With Dynamic Biosensors´ proFIRE® labelling kits, covalent conjugation of proteins to DNA can be carried out in a straightforward way with higher efficiency using lower amounts of protein.
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