Download 2022.3

Features

• Added plate plot
• Upgraded plot visualization to support plate plots
• Added vial plot selection for plate plot
• Added filtering options for plate plot
• Added hover effect for further inspection of each trace in plate plot
• Added auto-zoom logic for filtered series in plate plot
• Added axis scaling factor with synchronization for all traces in plate plot
• Added plate plot size adjustment depending on samples
• Upgraded automated analyses to use new blank referencing operation
• Added support for real-time referencing of multiple curves
• Added separate batch kinetics plots overview tab
• Added separate batch kinetics plots analyte tab

Improvements

• Improved performance of plots switching in the manual analysis
• Removed text indicators for old names of monophasic kinetics fits
• Updated switching frequency for predefined methods to 250 Hz
• Changed priming label in assay start wizard to “Initial Priming”
• Improved logic for sample tray position changes
• Improved assay search functionality
• Improved rate map icon
• Improved copying to clipboard functionality for plots
• Improved scrollbar experience for plot legends
• Improved export control for plots
• Added all kinetics fit model from the manual analysis in the automated analysis
• Upgraded names and descriptions of automated kinetics analyses
• Added description to real-time and blank referencing operations

Bug fixes (All reported crashes were fixed)

• Fixed empty composite method path labels
• Fixed lighter rate map series
• Fixed opening of XY-plots instead of rate map plot from the sidebar
• Fixed update of axes titles in rate map plot
• Fixed settings not opening by a double click on an analysis element
• Fixed fit of kinetics data with fewer than 5 points within their baseline
• Fixed copying an operation and creating align operation instead of pasting it
• Fixed unmasking operation in plots
• Fixed properties transfer from one assay to another during switching between them in the assay workflow
• Fixed parameter options for the change of values in composite methods
• Fixed buffer value after changing order of assays within an assay workflow
• Fixed composite method label for very large element sequences
• Fixed ignoring of description, scope, and group for creation of skippable element parameter
• Fixed incorrect unit forwarding from extract data operation
• Fixed disappearance of element headers in the manual analysis
• Fixed adjusting of assay list in assay work flow
• Fixed timeline tab not showing after live experiment was completed
• Fixed issue where measurements in spot 2 were shown when real-time referencing was not used
• Fixed empty parameter groups appearing despite all parameters being hidden
• Fixed number of points for baseline normalization not being available in real-time referencing operation
• Fixed miniature plot view disappearing after analysis recalculation
• Fixed issue with displaying category axis plots
• Fixed issue with incorrect view of empty composite flow diagram

Why can’t I see certain automated analyses options in the analyze pop-up anymore?

Because we’ve tightened the requirements for the automated kinetics analyses. If the data do not match the requirements for certain analyses they will not be displayed. We also changed the analyses names. “1:1” represents only monophasic fit models and since we’ve integrated the biphasic fit models in the automated analyses we had to remove it.

Here are the new analyses names and their purposes:

• 1:1 kinetics (with functionalization) -> Kinetics (with functionalization)
  Analysis extracting kinetics rate and equilibrium constants from real-time traces representing a single ligand-analyte-pair.

• 1:1 kinetics -> Kinetics
  Analysis extracting kinetics rate and equilibrium constants from real-time traces representing a single ligand-analyte-pair.

• 1:1 kinetics (FRET) -> Kinetics (FRET)
  Analysis extracting kinetics rate and equilibrium constants from real-time traces detected through fluorescence resonance energy transfer (FRET) and representing a single ligand-analyte-pair.

• Individual 1:1 kinetics -> Kinetics Screening
  Analysis extracting kinetics rate and equilibrium constants from real-time traces representing a multiple ligand-analyte-pairs.

• Multiplex 1:1 Kinetics -> Kinetics (Multiplex)
  Analysis extracting kinetics rate and equilibrium constants from real-time traces representing a multiple ligand-analyte-pairs recorded in parallel on multiple detection channels.

• New -> Kinetics (Batch)
  Analysis extracting kinetics rate and equilibrium constants from multiple batches of real-time traces, each representing a single ligand-analyte-pair.

Why can’t I open some of my saved analyses?

• With this release we’ve sharpened the rules of our analyses. Analyses that don’t meet the data requirements cannot be opened. Our Kinetics analysis (previously 1:1 Kinetics) can only analyze data from single ligand-analyte pair. Our Kinetics Screening (previously Individual 1:1 kinetics ) can now only analyze data from at least two analyte-ligand pairs. What you can do is analyze your data from your experiment again with the corresponding automated analysis.

Why can’t I directly change the curves color in the plate plot?

• During the research phase of the plate plot project, it was confirmed that a single color for all presented traces will be most suitable for the overview purposes. Nevertheless, you can still change the color of the traces. You need to disable the monochromatic colorization in the properties in order for the color changes to be visible. All changes that you make through the vector colorization tool within the XY-chart will be applied to the plate plot in the manual analysis.

Tipp: If you want to display all traces of the plot in a gradient format, click on one curve in the XY-Chart and press Ctrl+A on the keyboard to select all curves. After that select the gradient option and the color that you’d like from the vector colorization tool.