heliOS Software
Download 2022.3
We are pleased to announce that our latest 2022.3 release of heliOS is ready for you to download. Our main focus for this release was the evaluation of data. We’ve upgraded one of our analyses and introduced a new one. You’ll find a new data visualization mode, the plate plot. We also provide two extra operations, which make the manual data analysis much easier. As usual there are a couple of smaller improvements, which aim to increase the usability and understandability of our software. Let’s dive into the main features.

heliOS
version 2022.3.1 (124 MB)

heliOS Methods
version 2022.3 (7.9 MB)

heliX Firmware
version 6.284.3.1856 (1.5 GB)

heliX Quick Start Guide
version 5.1 (2.5 MB)
Note: Before running a new heliOS version, a firmware update of your heliX device is necessary.
SYSTEM REQUIREMENTS
Supported Operating System I Windows 10
heliOS requires the .NET 6 Desktop Runtime.
heliOS requires access to a postgreSQL database.
→ see install instructions
We are pleased to announce that our latest 2022.3 release of heliOS is ready for you to download. Our main focus for this release was the evaluation of data. We’ve upgraded one of our analyses and introduced a new one. You’ll find a new data visualization mode, the plate plot. We also provide two extra operations, which make the manual data analysis much easier. As usual there are a couple of smaller improvements, which aim to increase the usability and understandability of our software. Let’s dive into the main features.
1. Kinetics Screening analysis
Our Kinetics Screening is an upgrade of the previous 1:1 individual kinetics analysis. This analysis allows data evaluation of two or more analyte-ligand pairs. In the results overview you will find our new data visualization method, the plate plot. As the name suggests the data representation corresponds to the physical well plate. All traces will be displayed per well, independently of the plate size (96 or 384). If the samples are less than the total vials number, the plot will adjust automatically and display a smaller overview. With the plate plot you can quickly inspect the quality of the overall data. For your convenience we have added a focused view for each curve, which appears upon hover and allows you to zoom in and out. Both the plate plot and each individual curve can be exported. The individual kinetics results for each curve are displayed in a table, sorted by (well) position.
2. Plate plot in the manual analysis
Besides the Kinetics Screening overview, we have integrated the plate plot in our manual analysis. There, we offer additional filtering functionality for the data. This allows for either selection and removal of the data that is not suitable for further analysis or selection and retainment of the data that is of interest. Those options are revertible, and no data gets deleted during the process.
3. Kinetics (Batch)
Our new automated analysis is the Kinetics (Batch). Here we offer you the possibility to analyze multiple batches of real-time traces, each representing a single ligand-analyte pair. The results from the analysis are displayed with two separate tabs. Within the “Overview” you will find a separate plot per analyte and overall kinetics values. The “Analyte” tab allows you to navigate through your different analytes and inspect the kinetics for each curve withing the analysis.
4. Referencing operations
We have included the real-time referencing and the blank referencing in the manual analysis as extra operations. Both now compare the data in order to automatically pair curves. Before, real-time referencing could be accomplished by using the operations of normalizing, dividing, and then subtracting curves within the manual analysis. The extra operation can be now performed with a few clicks, allowing you to select the reference spot, the resulting unit and the points from start.
The Blank referencing operation, similarly to the real-time referencing was already possible in the manual analysis through addition of different smaller operations. Now you can easily choose one of the four modes, identical to the ones in the automated analyses. You can also remove unused blanks if you have any.
Features
- Added plate plot
- Upgraded plot visualization to support plate plots
- Added vial plot selection for plate plot
- Added filtering options for plate plot
- Added hover effect for further inspection of each trace in plate plot
- Added auto-zoom logic for filtered series in plate plot
- Added axis scaling factor with synchronization for all traces in plate plot
- Added plate plot size adjustment depending on samples
- Upgraded automated analyses to use new blank referencing operation
- Added support for real-time referencing of multiple curves
- Added separate batch kinetics plots overview tab
- Added separate batch kinetics plots analyte tab
Improvements
- Improved performance of plots switching in the manual analysis
- Removed text indicators for old names of monophasic kinetics fits
- Updated switching frequency for predefined methods to 250 Hz
- Changed priming label in assay start wizard to “Initial Priming”
- Improved logic for sample tray position changes
- Improved assay search functionality
- Improved rate map icon
- Improved copying to clipboard functionality for plots
- Improved scrollbar experience for plot legends
- Improved export control for plots
- Added all kinetics fit model from the manual analysis in the automated analysis
- Upgraded names and descriptions of automated kinetics analyses
- Added description to real-time and blank referencing operations
Bug fixes (All reported crashes were fixed)
- Fixed empty composite method path labels
- Fixed lighter rate map series
- Fixed opening of XY-plots instead of rate map plot from the sidebar
- Fixed update of axes titles in rate map plot
- Fixed settings not opening by a double click on an analysis element
- Fixed fit of kinetics data with fewer than 5 points within their baseline
- Fixed copying an operation and creating align operation instead of pasting it
- Fixed unmasking operation in plots
- Fixed properties transfer from one assay to another during switching between them in the assay workflow
- Fixed parameter options for the change of values in composite methods
- Fixed buffer value after changing order of assays within an assay workflow
- Fixed composite method label for very large element sequences
- Fixed ignoring of description, scope, and group for creation of skippable element parameter
- Fixed incorrect unit forwarding from extract data operation
- Fixed disappearance of element headers in the manual analysis
- Fixed adjusting of assay list in assay work flow
- Fixed timeline tab not showing after live experiment was completed
- Fixed issue where measurements in spot 2 were shown when real-time referencing was not used
- Fixed empty parameter groups appearing despite all parameters being hidden
- Fixed number of points for baseline normalization not being available in real-time referencing operation
- Fixed miniature plot view disappearing after analysis recalculation
- Fixed issue with displaying category axis plots
- Fixed issue with incorrect view of empty composite flow diagram
Why can’t I see certain automated analyses options in the analyze pop-up anymore?
Because we’ve tightened the requirements for the automated kinetics analyses. If the data do not match the requirements for certain analyses they will not be displayed. We also changed the analyses names. “1:1” represents only monophasic fit models and since we’ve integrated the biphasic fit models in the automated analyses we had to remove it.
Here are the new analyses names and their purposes:
- 1:1 kinetics (with functionalization) -> Kinetics (with functionalization)
Analysis extracting kinetics rate and equilibrium constants from real-time traces representing a single ligand-analyte-pair.
- 1:1 kinetics -> Kinetics
Analysis extracting kinetics rate and equilibrium constants from real-time traces representing a single ligand-analyte-pair.
- 1:1 kinetics (FRET) -> Kinetics (FRET)
Analysis extracting kinetics rate and equilibrium constants from real-time traces detected through fluorescence resonance energy transfer (FRET) and representing a single ligand-analyte-pair.
- Individual 1:1 kinetics -> Kinetics Screening
Analysis extracting kinetics rate and equilibrium constants from real-time traces representing a multiple ligand-analyte-pairs.
- Multiplex 1:1 Kinetics -> Kinetics (Multiplex)
Analysis extracting kinetics rate and equilibrium constants from real-time traces representing a multiple ligand-analyte-pairs recorded in parallel on multiple detection channels.
- New -> Kinetics (Batch)
Analysis extracting kinetics rate and equilibrium constants from multiple batches of real-time traces, each representing a single ligand-analyte-pair.
Why can’t I open some of my saved analyses?
- With this release we’ve sharpened the rules of our analyses. Analyses that don’t meet the data requirements cannot be opened. Our Kinetics analysis (previously 1:1 Kinetics) can only analyze data from single ligand-analyte pair. Our Kinetics Screening (previously Individual 1:1 kinetics ) can now only analyze data from at least two analyte-ligand pairs. What you can do is analyze your data from your experiment again with the corresponding automated analysis.
Why can’t I directly change the curves color in the plate plot?
- During the research phase of the plate plot project, it was confirmed that a single color for all presented traces will be most suitable for the overview purposes. Nevertheless, you can still change the color of the traces. You need to disable the monochromatic colorization in the properties in order for the color changes to be visible. All changes that you make through the vector colorization tool within the XY-chart will be applied to the plate plot in the manual analysis.
Tipp: If you want to display all traces of the plot in a gradient format, click on one curve in the XY-Chart and press Ctrl+A on the keyboard to select all curves. After that select the gradient option and the color that you’d like from the vector colorization tool.
In case you need help, please do not hesitate to contact our support team at support@dynamic-biosensors.com.
INSTALL INSTRUCTIONS
We recommend to always use the newest versions of heliOS and heliX firmware.
Please update first your heliX device with the latest firmware version before installing a new version of heliOS.
SYSTEM REQUIREMENTS
Supported Operating System I Windows 10
heliOS requires the .NET 6 Desktop Runtime.
heliOS requires access to a postgreSQL database.
heliX FIRMWARE UPDATE
Note: The installation of a new heliX firmware version must be performed from a computer which is connected to the heliX device.
Note: Skip this step if you are only interested in updating the heliOS version on a computer which is not used to control the heliX device.
- Ensure that the heliX device is switched on and connected to the control PC (directly or via network).
- Download the above listed version of the heliX firmware. We recommend to always use the newest version.
- Run the EXE installer on the control PC and follow the setup steps. You can find the IP address of your heliX in heliOS under Devices.
After approx. 10 minutes the heliX will be restarted and ready to use.
INSTALLATION OF heliOS
Note: If heliOS is installed for the first time on a computer you need to first install the .NET 6 Desktop Runtime (see Installation of Microsoft .NET 6) and a PostgreSQL database (see Installation and setup of postgreSQL database).
- Download the above listed version of heliOS. We recommend to always use the newest version.
- Run the MSI installer and follow the setup steps.
- The first time you open heliOS on a computer you need to enter your license key (Settings → License).
Copy and paste the license key into the intended input field and confirm. - In addition to 3, the first time you are using heliOS you need to enter your database access data (Settings → Database).
- Now heliOS is ready to use and can, for example, be connected to your heliX device.
IMPORT OF heliOS METHODS
- Download the above listed heliOS methods.
- Unzip the downloaded file once. The unzipped folder contains a range of zipped heliOS methods. Do not unzip these files.
- Open heliOS and go to “Methods”.
- Click on “Import” in the top menu and select one or several heliOS methods (ZIP files) from the unzipped folder.
- Click on “Open”. Now your data base is equipped with the newest heliOS methods.
INSTALLATION OF MICROSOFT .NET 6
Note: heliOS requires the .NET 6 Desktop Runtime on your Windows operating system. If already installed you can skip this step.
- Download the .NET 6 Desktop Runtime installation file (64-bit version).
- Run the EXE installer and follow the setup steps.
- After the installation process has finished you are ready to install heliOS.
INSTALLATION AND SETUP OF A postgreSQL DATABASE
Note: heliOS requires a postgreSQL database either on a server in your local network or on your Windows operating system. If you do not have access to a network-based postgreSQL database you need to install one on your local computer. If already installed you can skip this step.
- Download postgreSQL from the link below. Select version 12.X for Windows x86-64.
https://www.enterprisedb.com/downloads/postgres-postgresql-downloads - Run the EXE installer and follow the setup steps. A detailed installation guide can be found here: https://www.enterprisedb.com/docs/supported-open-source/postgresql/installer/02_installing_postgresql_with_the_graphical_installation_wizard/01_invoking_the_graphical_installer/
If you need help with setting up the postgrSQL database please ask your system administrator or contact our support team at support@dynamic-biosensors.com. - After the installation process has finished you are ready to install heliOS.
In case you need help, please do not hesitate to contact our support team at support@dynamic-biosensors.com.
Questions?
Contact Us
Our support team is happy to help.