heliOS Software

Download 2022.4

We are excited to announce our last release 2022.4 for this year. We have launched our new RT-IC (Real-Time Interaction Cytometry) technology (for more information → click here), which has quite the impact on the heliOS software. We have designed new methods specifically for RT-IC measurements. You can find the download instructions below. Furthermore, we are introducing with this release two new automated analyses and one new operation. We improved the plots, fits and experiment image handling as well. As usual there are a couple of smaller improvements, which aim to increase the usability and understandability of our software. 

heliOS

version 2022.4.0
(122 MB)

DOWNLOAD

heliOS Methods
for switchSENSE®

version 2022.4 (8 MB)

DOWNLOAD

heliOS Methods
for RT-IC

version 2022.4 (0.5 MB)

DOWNLOAD

heliX Firmware

version 6.307.3.1926
(1.5 GB)

DOWNLOAD

heliX Quick Start
Guide

version 6.1 (2.5 MB)

DOWNLOAD

Note: Before running a new heliOS version, a firmware update of your heliX or heliXcyto device is necessary.

SYSTEM REQUIREMENTS
Supported Operating System I Windows 10

heliOS requires the .NET 6 Desktop Runtime. 
heliOS requires access to a postgreSQL database.

→ see install instructions

OTHER RELEASES

We are excited to announce our last release 2022.4 for this year. We have launched our new RT-IC (Real-Time Interaction Cytometry) technology (for more information → click here), which has quite the impact on the heliOS software. We have designed new methods specifically for RT-IC measurements. You can find the download instructions below. Furthermore, we are introducing with this release two new automated analyses and one new operation. We improved the plots, fits and experiment image handling as well. As usual there are a couple of smaller improvements, which aim to increase the usability and understandability of our software. 

Kinetics (RT-IC) 

Detailed information about the RT-IC (Real-Time Interaction Cytometry) technology can be found here. The first automated analysis that we’ve introduced is the Kinetics (RT-IC). The overview of the results is very similar to the Kinetics (Batch) analysis. There are two tabs, one that serves as an overview of the whole experiment and a second one that shows the different runs. Both tabs present the most important kinetics values. The plot titles represent the cell type and the analyte. Those correspond to the input fields of the assay workflow “Analyte name” and “Cell name”.  

Dissociation Kinetics (RT-IC) 

When measuring interactions in living cells, you can also analyze only the dissociation of your dataset. You can use Dissociation Kinetics (RT-IC) analysis for that purpose. The results of this analysis follows the logic of the Kinetics (RT-IC) analysis.  

Export of multiple plots 

As part of the automated RT-IC analyses, we’ve integrated an export option for multiple plots. You can now export/copy to clipboard all plots from the overview tab of the analysis. If you use the copy to clipboard functionality and paste the plots in PowerPoint, they will be displayed as single element/group. But you can ungroup them and adjust them individually depending on your needs. 

Chronological alignment 

This new manual analyses operation allows you to display the curves in a chronological order, starting with the earliest data recorded. 

Image handling 

We have introduced a couple of new features for the images of the experiments. Now you can view the images in full-screen size via the slide show mode. Multiple exporting options are available. You can export a single image, multiple preselected images, or all available images in one go. For your convenience, we designed the image label as following “run name_description”. 

Series properties 

We changed the access of the series properties for the plot curves. Previously accessible through a double click, now you can access them using one of the buttons on the top left. This applies for the selection of a single curve, if multiple curves are selected, the same button will open the vector colorization tool. 

Features 

  • Added automated Kinetics and Dissociation Kinetics analyses for RT-IC measurements 
  • Added cell capture and regeneration measurements to manual analyses behind automated RT-IC analyses 
  • Added real-time referencing operation for RT-IC measurements 
  • Added chronological alignment to RT-IC automated analyses 
  • Added custom trace coloring to RT-IC analyses 
  • Added association concentrations in plot legends for RT-IC automated analyses  
  • Added “Specifics” tab with fit and individual results to the RT-IC automated analyses 
  • Added cell and analyte label to plots and individual results tables in automated RT-IC analysis 
  • Added export option (png; svg) for multiple plots for the RT-IC automated analyses 
  • Added separate Real-time-Referencing operation for RT-IC measurements 
  • Added possibility to prime with custom maintenance buffers 
  • Added new operation for chronological alignment of curves in the manual analyses 
  • Added handling of association-only curves to full-range kinetics fits 
  • Added handling of titration kinetics data to all full-range kinetics fits 
  • Added a slide show mode to display the experiment images 
  • Added export of multiple preselected experiment images 
  • Added export of all experiment images at once 

Improvements 

  • Added heliXcyto case to heliOS device control screen 
  • Updated heliX chip type for chip tray view 
  • Added dedicated renderings for heliX+ devices 
  • Added support of working volume to define vial function 
  • Improved access to single series properties 
  • Improved chip plastic color display as read from heliX
  • Improved chip types display in chip tray view 
  • Added ability for Passivation to be automatically distributed to small vials 
  • Improved rate map points opacity 
  • Improved plots colorization 
  • Updated heliX chip names 
  • Improved series properties accessing with dynamic icons and labels  
  • Improved display of long buffer names 
  • Updated label of the scratchpad context menu to Smart Operation 

      Bug fixes

      • Fixed update of assay parameters displayed as run parameters 
      • Fixed vector properties panel displaying far too tall 
      • Fixed incorrect deletion behavior from scratch pad analysis context menu 
      • Fixed issue with incorrectly resizing of plot properties panel 
      • Fixed Kinetics analysis not being available for kinetics test experiments 
      • Fixed concentration not showing on a scratch-pad element 
      • Fixed plot export context menu labeling 
      • Fixed rate map isolines disappearing after property changes 
      • Fixed issue where ligand changes are not taken into account when separating batches in Kinetics Batch Analysis 
      • Fixed timeline tab not showing after live experiment was completed 
      • Fixed random analysis element headers disappearance 
      • Fixed adjusting of assay list in assay work flow  
      • Fixed re-aggregation failures on imported experiments 
      • Fixed data filtering for aliases  
      • Fixed plot name not showing up in plot panes 

            Where can I find RT-IC assays? 

            • Assays specifically designed for cell measurements with the heliXcyto device can be found under the custom assays of the adding assay window. They are easy to recognize by the RT-IC (Real-Time Interaction Cytometry) tag.

               

            Where can I find further information about fitting operations? 

            • You can find more information about the fitting operations in chapter 5.3 “Kinetics fit operations” of the heliX Quick Start Guide (→DOWNLOAD). 

            How can I remove spikes from my RT-IC data/plot? 

            • To remove spikes and generally to adjust your data, you need to navigate to the Scratchpad. Create a plot from the last performed operation (gray element) before the fitting (blue element). Use the “mask points” functionality to select data points that seem to distort your results. Selected points will be removed from the fit. When you navigate back to the plot of the automated analysis, you’ll notice that it also has changed. Be careful with the specifics tab in RT-IC Kinetics analyses, since the kinetics values of the new fit will not be represented there. You can copy those from the scratchpad.

               

             

            In case you need help, please do not hesitate to contact our support team at support@dynamic-biosensors.com.

            INSTALL INSTRUCTIONS

            We recommend to always use the newest versions of heliOS and heliX firmware.
            Please update first your heliX device with the latest firmware version before installing a new version of heliOS.

            SYSTEM REQUIREMENTS

            Supported Operating System I Windows 10

            heliOS requires the .NET 6 Desktop Runtime.
            heliOS requires access to a postgreSQL database.

            heliX FIRMWARE UPDATE

            Note: The installation of a new heliX firmware version must be performed from a computer which is connected to the heliX device.

            Note: Skip this step if you are only interested in updating the heliOS version on a computer which is not used to control the heliX device.

            1. Ensure that the heliX device is switched on and connected to the control PC (directly or via network).
            2. Download the above listed version of the heliX firmware. We recommend to always use the newest version.
            3. Run the EXE installer on the control PC and follow the setup steps. You can find the IP address of your heliX in heliOS under Devices.
              After approx. 10 minutes the heliX will be restarted and ready to use.

            INSTALLATION OF heliOS

            Note: If heliOS is installed for the first time on a computer you need to first install the .NET 6 Desktop Runtime (see Installation of Microsoft .NET 6) and a PostgreSQL database (see Installation and setup of postgreSQL database).

             

            1. Download the above listed version of heliOS. We recommend to always use the newest version.
            2. Run the MSI installer and follow the setup steps.
            3. The first time you open heliOS on a computer you need to enter your license key (Settings → License).
              Copy and paste the license key into the intended input field and confirm.
            4. In addition to 3, the first time you are using heliOS you need to enter your database access data (Settings → Database).
            5. Now heliOS is ready to use and can, for example, be connected to your heliX device.

            IMPORT OF heliOS METHODS

            1. Download the above listed heliOS methods.
            2. Unzip the downloaded file once. The unzipped folder contains a range of zipped heliOS methods. Do not unzip these files.
            3. Open heliOS and go to “Methods”.
            4. Click on “Import” in the top menu and select one or several heliOS methods (ZIP files) from the unzipped folder.
            5. Click on “Open”. Now your data base is equipped with the newest heliOS methods.

            INSTALLATION OF MICROSOFT .NET 6

            Note: heliOS requires the .NET 6 Desktop Runtime on your Windows operating system. If already installed you can skip this step.

             

            1. Download the .NET 6 Desktop Runtime installation file (64-bit version).
            2. Run the EXE installer and follow the setup steps.
            3. After the installation process has finished you are ready to install heliOS.

            INSTALLATION AND SETUP OF A postgreSQL DATABASE

            Note: heliOS requires a postgreSQL database either on a server in your local network or on your Windows operating system. The heliX control computer already comes with a set up postgreSQL database. Continue only if you want to install a second postgreSQL database on another computer.

            1. Download postgreSQL from the link below. Select version 12.X for Windows x86-64.
              https://www.enterprisedb.com/downloads/postgres-postgresql-downloads
            2. Run the EXE installer and follow the setup steps. Use the default settings and enter a password of your choice. We recommend to use the same password as on the heliX control computer (mypassword). Do not launch the Stack Builder at the end of the installation wizard.
            3. After the installation of postgreSQL open the Windows Run app (or press the Windows+R keys on the keyboard).
            4. Enter the following command line into the field and confirm: c:\Program Files\PostgreSQL\12\bin\createdb.exe -U postgres heliOS

            5. You now will be asked for a password. Enter the password you selected during the postgreSQL installation and press Enter. If you will be asked again for the password, enter it again and confirm with the Enter key.
            6. Repeat step 3 and enter the following command line into the field. Confirm afterwards: c:\Program Files\PostgreSQL\12\bin\createuser.exe -P -Upostgres heliOS

            7. Repeat step 5 (Enter the password).
            8. Now, you can start heliOS with the following database credentials (Settings → Database):
              Host: localhost | Database: heliOS | User: heliOS | Password: [your password]

            9. Enter your heliOS license key (Settings → License) if necessary.

              If you need help with setting up the postgrSQL database please ask your system administrator or contact our support team at support@dynamic-biosensors.com.

              In case you need help, please do not hesitate to contact our support team at support@dynamic-biosensors.com.

              Questions?
              Contact Us

              Our support team is happy to help. 

              support@dynamic-biosensors.com

              +49 89 89 74 544-0

              Perchtinger Str. 8/10, 81379 München, Germany

              M-F: 8am-5pm, S-S: Closed