The Adapter Biochip. 

One chip. One workflow. 
Many ways to functionalize the surface. 

Adapter Biochip Design

heliX®  adapter chip – 2 spots with 2 different anchor sequences for DNA-encoded adressing. 

Adapter Biochip and suitable Kits

Browse through our product list of the heliX®  adapter biochip and suitable kits. 

Sensor Functionalization

heliX® chip and sensor functionalization must be so routine that your mind is free to focus on your samples. 

Chip Apps

Download our apps to create your own custom heliX® biochip and read-out the status and usage history of any heliX® chip with your mobile device.

Adapter Biochip Design. 

2 spots with 2 different anchor sequences for DNA-encoded addressing. 

The heliX® adapter biochip features 2 spots (4 signals)


Spot 1
: measurement spot in red and green 
Spot 2: reference / measurement spot in red and green

Anchor Strands, fixed

The heliX® chip surface is modified with covalently attached single stranded DNA Anchor strands, which have been optimized to bind Adapter strands and Ligand strands with ultimate specificity and stability. The 48-mers are carefully selected and remain double-stranded up to 80°C.

Ligand strand, exchangeable

Your molecule of interest (the ligand) is immobilized onto the surface by the DNA nanolever, either by direct covalent coupling or by tag-capturing methodsCoupling of proteins or small molecules to Ligand strands is readily performed using easy-to-use coupling kits.

 

Adapter strand, exchangeable

Adapter strands give you maximal experimental flexibility: use a standard adapter for most binding and conformation applications, or pick a long DNA-origami nanostructure to size the largest molecular complexes. Dyes are exchangeable, too.

Sensor Functionalization.

heliX® chip and sensor functionalization must be so routine that your mind is free to focus on your samples

Automated sensor exchange

Load up to 5 chips in one rack and switch automatically between 5 channels and 10 sensor spots.

 

RFID near field communication

between instrument and chip for seamless traceability of chip status and usage history.
Ideal for quality-controlled GxP environments and consistent, error-free results.


DOWNLOAD the heliX chip app to read-out the status and usage history of any heliX® chip with your mobile device.

 

Robust microfluidics

Simplistic single-channel design, made from durable glass, withstanding highest flow rates and corrosive chemicals, disposable.

 

2 detection spots x 2 colors

For real-time referenced measurements of 2 interactions, or monitoring of up to 4 interactions simultaneously.

Sensor functionalization through standard,
automated workflows.

Sensor functionalization must be so routine that your mind is free to focus on your samples.
The switchSENSE®  workflow is simple, reliable, and effective:

→ click on the picture to see a larger version of it. 

Ligand immobilization
Ligand coupling is carried out in-vitro for superior purity.

Low sample consumption
5 pmol in 20 µL adapter-ligand conjugate per immobilization.

Fast
Wash off analyte/ligand within 1 second.
Immobilize fresh ligand within 1 to 5 minutes.

Fresh ligand, every time
For reproducible results, every time. DNA-mediated functionalization eliminates the need to scout for regeneration conditions.
Why not have a coffee instead?

Repeated chip regeneration
Confidence in chip quality over more than 50 cycles*.

Economics
Less than $2 per datapoint for chip and reagent costs*.

* Numbers reflect typical workflows and can vary depending on experimental conditions.

Molecular lego, basically. 
Working with Adapter strands

Reporter fluorophore

Our standard red and green fluorophores work for almost any application, reporting slightest changes in its molecular environment with high sensitivity. But experimental creativity cannot be predicted, so pick your optimal probe from a wide range of chemically diverse dyes. Rhodamines, cyanines, oxazines, acrinidines and others are available on stock and custom synthesis is supported, too… your choice. heliOS software supports pre-configured screening workflows to identify your preference in green and red with little effort.

Easy to adapt

Kits with ready-to-use solutions and pre-configured heliOS software workflows.

Standard adapter

A single-stranded sequence with 96 bases, modified with a reporter dye in green or red. The upper 48 bases bind the ligand strand. Sequence information is openly available, in case you’d like to custom-make your ligand strands instead of using a DBS coupling kit.

 
DNA nanostructures: Think big or small. Flexible or rigid. Mono or bi-specific.

Standard heliX® chips can be combined with DNA nanostructures such as flexible, antibody-like Y-structures presenting two ligands. Or rigid 50 or 100 nm long DNA-origami nanolevers to actuate and size large molecular complexes.

Ligand strands are compatible with standard adapters as well as with DNA nanostructures. Couple ligands just once and run assays with different adapters without additional effort.

heliX® Adapter Biochip.
How to functionalize the surface with ligands of interest in 3 simple steps.

→ click on the picture to see a larger version of it. 

MIX and RUN!

Ligand density control made easy on a heliX® chip.

Adjusting different ligand densities on a heliX® Adapter Chip is easier than preparing a decent Vodka Martini:

  1. Mix ligand and adapter strands at the desired ratio.
  2. Shake or stir, it doesn’t really matter. Place vial in autosampler.

heliX® Biochip Apps

Free apps that will make your work in the lab easier

heliX® MIX&RUN.
Create your own custom heliX
®
biochip to meet your experimental needs. 

The heliX® MIX&RUN app determines the volumes of your ligand(s) and Adapter-dye strands in order to create a biochip surface with your desired ligand densities. 

Use the heliX® chip app to read 
the status of your biochips.

Scan biochips with your smartphone.

The heliX chip app allows you to read out the status and usage history of any heliX® chip with your mobile device.

Chip Scanner

Identify the chip type and ID.
Check the date of first use and the status of your chip.
Verify the expiration date.
How many hours has the chip been in use?
How many times has it been regenerated?
What temperatures had the chip been exposed to?

Specification Sheet

Download the spec sheet for more information.

Compatible Kits

Directly access a list of compatible coupling and adapter kits.

Contact Us

Contact a specialist for technical support and assay development. 

Order Chip

Conveniently place an order.

 

heliX® Biochips

Browse through our Adapter Chip & Adapter Chip Kits product list. 

Order No.  Information
 
heliX® Adapter Biochips
 

Chip with 2 spots; Anchor strand 1 immobilized on spot 1,
Anchor strand 2 immobilized on spot 2; compatible with all
heliX® Adapter strands and heliX® coupling kits.
Including Test and standby solution.

ADP-48-2-0 Pack of 1
ADP-48-2-0_3 Pack of 3
ADP-48-2-0_5 Pack of 5
   
 
Adapter Kits – suitable for heliX® Adapter Biochips
ASP-1-Ra

Adapter strands with red dye Ra package including
Adapter strand 1
with red dye Ra and
Adapter strand 2 with red dye Ra prehybridized with Ligand-free strand.
5 aliquots each for 50 regenerations.

ASP-1-Ga Adapter strands with green dye Ga package including
Adapter strand 1
with green dye Ga and
Adapter strand 2 with green dye Ga prehybridized with Ligand-free strand.
5 aliquots each for 50 regenerations.
   
TS-0

Test and standby solution
Ready to use mix of AS-1-Ra-lfs and AS-2-Ra-lfs
Concentration: each 100 nM; Volume: 5 x 100 µl

   
AS-1-Ra Adapter strand 1 with red dye Ra (recommended)
5 aliquots for 50 regenerations
AS-1-Rb Adapter strand 1 with red dye Rb 
5 aliquots for 50 regenerations
AS-1-Rc Adapter strand 1 with red dye Rc
5 aliquots for 50 regenerations
AS-1-Ga Adapter strand 1 with green dye Ga (recommended)
5 aliquots for 50 regenerations
AS-1-Gb Adapter strand 1 with green dye Gb
5 aliquots for 50 regenerations
AS-1-Gc Adapter strand 1 with green dye Gc
5 aliquots for 50 regenerations
   
AS-2-Ra Adapter strand 2 with red dye Ra (recommended)
5 aliquots for 50 regenerations
AS-2-Rb Adapter strand 2 with red dye Rb
5 aliquots for 50 regenerations
AS-2-Rc Adapter strand 2 with red dye Rc
5 aliquots for 50 regenerations
AS-2-Ga Adapter strand 2 with green dye Ga (recommended)
5 aliquots for 50 regenerations
AS-2-Gb

Adapter strand 2 with green dye Gb
5 aliquots for 50 regenerations

AS-2-Gc

Adapter strand 2 with green dye Gc
5 aliquots for 50 regenerations

   
 AS-2-Ga-lfs

Adapter strand 2 with green dye Ga – prehybridized with Ligand-free strand
5 aliquots for 50 regenerations 

 AS-2-Gb-lfs  Adapter strand 2 with green dye Gb – prehybridized with Ligand-free strand
5 aliquots for 50 regenerations
AS-2-Gc-lfs

Adapter strand 2 with green dye Gc – prehybridized with Ligand-free strand
5 aliquots for 50 regenerations

AS-2-Ra-lfs

Adapter strand 2 with red dye Ra – prehybridized with Ligand-free strand
5 aliquots for 50 regenerations

AS-2-Rb-lfs

Adapter strand 2 with red dye Rb – prehybridized with Ligand-free strand
5 aliquots for 50 regenerations

AS-2-Rc-lfs

Adapter strand 2 with red dye Rc – prehybridized with Ligand-free strand
5 aliquots for 50 regenerations

 

 

DS-6

Dye scouting kit with 6 different dyes (Ga, Gb, Gc, Ra, Rb and Rc)
for Adapter strand 1 and 2; Adapter strand 2 is prehybridized with Ligand-free strand;
Content: AS-1-Ra, AS-1-Rb, AS-1-Rc, AS-1-Ga, AS-1-Gb, AS-1-Gc, AS-2-Ra-lfs, AS-2-Rb-lfs, AS-2-Rc-lfs, AS-2-Ga-lfs, AS-2-Gb-lfs, AS-2-Gc-lfs.
One aliquot each for 10 regnerations. 

DC-0

Density control kit : complementary Anchor strand 1 (cAnchor 1) and 
complementary Anchor strand 2 (cAnchor 2)
Concentration: 200 nM; Volume: 5 x 200 µl cAnchor1 and 5 x 200 µl cAnchor2

LFS-0

Ligand-free strand
Concentration: 500 nM; Volume: 5 x 100 µl

Performance verified chips for use in QC and GxP experiments

All biochips are available as performance verified versions upon request. Performance verified chips are especially selected and each detection spot is extensively tested under application conditions and QC approved.

Comprehensive QC documentation of performance indicators is provided with every chip for consistent traceability of results. Recommended for use in QC or GxP environments. Please contact order@dynamic-biosensors.com for details.

Edit
Order No. Information
Cleaning Chip
CLC-0 Chip for cleaning purposes with microfluidics, without DNA recommended for 100 cleaning cycles.
Custom Biochips

Custom biochips with tailor-made surface modifications are available upon request.
Please contact order@dynamic-biosensors.com and describe your application.

A chip specialist will get in touch with you shortly to discuss technical requirements, solutions, and provide you with timeline and cost estimates.

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