switchSENSE®

Solutions for research & development. 

Sensor Functionalization

heliX®  adapter design. One chip. One workflow. Many ways to functionalize the surface.

Starter Packs

Starter packs are ideal to get you up and running quickly on your first experiments.

Buffers & Solutions

We offer a range of standard buffers and solutions optimized for switchSENSE® experiments.

Coupling & Capture Kits

To link a Ligand strand onto the complementary DNA strand, we offer a range of coupling kits.

Tutorials & Test Kits

Tutorial & Test kits are ideal for teaching or revision.

Consumables

All the necessary glassware, vials, well plates and other consumables you may need for your switchSENSE® studies.

Sensor functionalization

through standard, automated workflows.

heliX® chip functionalization.
A robust way of tethering molecules to a surface sensor.

DNA duplexes are forming, separating, and re-forming successfully since first life forms appeared on earth. 3.7 billion years later we are using DNA helix formation to zip molecules onto sensor spots. Sorry for taking so long, but we wanted to be sure it works just fine.

heliX® chip with DNA Anchor Strands, fixed

The heliX® chip surface is modified with covalently attached single stranded DNA Anchor strands, which have been optimized to bind Adapter strands and Ligand strands with ultimate specificity and stability. The 48-mers are carefully selected and remain double-stranded up to 80°C.

Ligand strand, exchangeable

Your molecule of interest (the ligand) is immobilized onto the surface by the DNA nanolever, either by direct covalent coupling or by tag-capturing methodsCoupling of proteins or small molecules to Ligand strands is readily performed using easy-to-use coupling kits.

 

Adapter strand, exchangeable

Adapter strands give you maximal experimental flexibility: use a standard adapter for most binding and conformation applications, or pick a long DNA-origami nanostructure to size the largest molecular complexes. Dyes are exchangeable, too.

Molecular lego, basically. 
Working with Adapter strands

Reporter fluorophore

Our standard red and green fluorophores work for almost any application, reporting slightest changes in its molecular environment with high sensitivity. But experimental creativity cannot be predicted, so pick your optimal probe from a wide range of chemically diverse dyes. Rhodamines, cyanines, oxazines, acrinidines and others are available on stock and custom synthesis is supported, too… your choice. heliOS software supports pre-configured screening workflows to identify your preference in green and red with little effort.

Easy to adapt

Kits with ready-to-use solutions and pre-configured heliOS software workflows.

Standard adapter

A single-stranded sequence with 96 bases, modified with a reporter dye in green or red. The upper 48 bases bind the ligand strand. Sequence information is openly available, in case you’d like to custom-make your ligand strands instead of using a DBS coupling kit.

 
DNA nanostructures: Think big or small. Flexible or rigid. Mono or bi-specific.

Standard heliX® chips can be combined with DNA nanostructures such as flexible, antibody-like Y-structures presenting two ligands. Or rigid 50 or 100 nm long DNA-origami nanolevers to actuate and size large molecular complexes.

Ligand strands are compatible with standard adapters as well as with DNA nanostructures. Couple ligands just once and run assays with different adapters without additional effort.

Sensor functionalization through standard,
automated workflows.

Sensor functionalization must be so routine that your mind is free to focus on your samples.
The switchSENSE®  workflow is simple, reliable, and effective:

→ click on the picture to see a larger version of it. 

Ligand immobilization
Ligand coupling is carried out in-vitro for superior purity.

Low sample consumption
5 pmol in 20 µL adapter-ligand conjugate per immobilization.

Fast
Wash off analyte/ligand within 1 second.
Immobilize fresh ligand within 1 to 5 minutes.

Fresh ligand, every time
For reproducible results, every time. DNA-mediated functionalization eliminates the need to scout for regeneration conditions.
Why not have a coffee instead?

Repeated chip regeneration
Confidence in chip quality over more than 50 cycles*.

Economics
Less than $2 per datapoint for chip and reagent costs*.

* Numbers reflect typical workflows and can vary depending on experimental conditions.

heliX® Adapter Biochip.
How to functionalize the surface with ligands of interest in 3 simple steps.

→ click on the picture to see a larger version of it. 

heliX® MIX&RUN.
Create your own custom heliX
®
biochip to meet your experimental needs. 

The heliX® MIX&RUN app determines the volumes of your ligand(s) and Adapter-dye strands in order to create a biochip surface with your desired ligand densities. 

heliX® biochip.
Exchangeable ligand strand

Covalent Coupling vs. Tag Capture Methods. 

→ click on the picture to see a larger version of it. 

Reagents.

COUPLING & CAPTURE KITS.

To link a Ligand strand onto the complementary DNA strand, we offer a range of coupling kits.
In case you have any questions, please contact info@dynamic-biosensors.com.

 

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Order No. Information
 
Amine Coupling Kits
HK-NHS-1

heliX® Amine Coupling Kit 1
Functionalization of the Ligand strand with biomolecules containing a primary amine, 5 different conjugations (proFIRE® purification)

HK-NHS-2

heliX® Amine Coupling Kit 2
Functionalization of the Ligand strand with biomolecules containing a primary amine, 5 different conjugations (spin column purification)

HK-NHS-3

heliX® Amine Coupling Kit 3
Functionalization of the Ligand strand with low pI (pI < 6) biomolecules containing a primary amine, 5 different conjugations (proFIRE® purification)

Thiol Coupling Kits
HK-MAL-1

heliX® Thiol Coupling Kit 1

Functionalization of the Ligand strand with biomolecules containing a free thiol group, 5 different conjugations (proFIRE® purification)
HK-MAL-2

heliX® Thiol Coupling Kit 2
Functionalization of the Ligand strand with biomolecules containing a free thiol group, 5 different conjugations (spin column purification)

Streptavidin Capture Kit
HK-BIO-1
Ready-to-use Adapter strand 1-Ra prehybridized with Ligand strand modified with Biotin. Including Adapter strand 2-Ra prehybridized with Ligand-free strand. For 20 regenerations.
His Capture Kit
HK-NTA-1

Ready-to-use Adapter strand 1-Ra prehybridized with Ligand strand modified with Tris-NTA. Including Adapter strand 2-Ra prehybridized with Ligand-free strand. For 20 regenerations.

 

Biotin Capture Kit / Twin-Strep-tag Capture Kit
HK-SA-1

Biotin Capture Kit
Ready-to-use Adapter strand 1-Ra prehybridized with Ligand strand modified with Streptavidin. Including Adapter strand 2-Ra prehybridized with Ligand-free strand. For 20 regenerations.

HK-SXT-1

Twin-Strep-tag Capture Kit
Ready-to-use Adapter strand 1-Ra prehybridized with Ligand strand modified with Strep-Tactin®XT*. Including Adapter strand 2-Ra prehybridized with Ligand-free strand. For 20 regenerations.

*Strep-Tactin®XT is a product of IBA GmbH.
Fc Capture Kits
HK-FC-1

Fc Capture Kit A
Ready-to-use Adapter strand 1-Ra prehybridized with Ligand strand modified with Protein A. Including Adapter strand 2-Ra prehybridized with Ligand-free strand. For 20 regenerations.

HK-FC-2

Fc Capture Kit G
Ready-to-use Adapter strand 1-Ra prehybridized with Ligand strand modified with Protein G. Including Adapter strand 2-Ra prehybridized with Ligand-free strand. For 20 regenerations.

GFP Capture Kit
HK-GFP-1
Ready-to-use Adapter strand 1-Ra prehybridized with Ligand strand modified with GFP binding protein*. Including Adapter strand 2-Ra prehybridized with Ligand-free strand. For 20 regenerations.

*GFP / GST binding protein is a product of ChromoTek GmbH.

Reducing Agent Kit 1 for Proteins and Antibodies

RK-PA-1

Chemical reduction of disulfide bonds with TCEP.

 

STARTER PACKS.

Starter packs are ideal to get you up and running quickly on your first experiments. Supplied with a detailed user’s manual and reagents they will guide you through a full experiment. 

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Order No. Information
Starter Packs
SP-HLX-1

heliX® and proFIRE® Starter Pack 1
Contains 18 products to get you up and running quickly on your first experiments: 1 x ADP-48-2-0, 1 x CLC-0, 1 x WP-096-650-8, 1 x WP-SEAL-HLX, 1 x AV-100-100, 1 x AV-015-100N, 1 x TB-CC-1-1, 1 x SY-250-1, 1 x BU-P-150-10, 1 x BU-P-1000-5, 1 x BU-PE-140-10, 1 x SOL-PAS-1-5, 1 x SOL-REG-12-1, 1 x HK-NHS-1, 1 x DK-KT-1, 1 x DK-FT-KT-1, 1 x ASP-1-Ra, 1 x TS-0

SP-HLX-2

heliX® Starter Pack 2
Contains 14 products to get you up and running quickly on your first experiments: 1 x ADP-48-2-0, 1 x CLC-0, 1x WP-096-650-8, 1x WP-SEAL-HLX, 1x AV-100-100, 1 x AV-015-100N, 1x BU-PE-140-10, 1x SOL-PAS-1-5, 1x SOL-REG-12-1, 1x HK-NHS-2, 1 x DK-KT-1, 1 x DK-FT-KT-1, 1 x ASP-1-Ra, 1 x TS-0

TUTORIALS & TEST KITS.

Tutorial & Test kits are ideal for teaching or revision. Supplied with a detailed user’s manual and reagents they will guide users through a full experiment. 

In general, we recommend an annual service visit, which includes a complete documented re-qualification of the instrument according to QC standards.

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Order No. Information
 
Test Kits
DK-FT-KT-1

Fluidic and Kinetic Test Kit
All necessary solutions to test the fluidic components of the heliX® instrument including a kinetic test measurement. Includes 3 reactions.

Tutorial Kits
DK-KT-1
Basic Binding Kinetics Tutorial
This tutorial enables you to conduct a basic kinetic experiment using nucleic acids. Includes 3 reactions.
DK-DP-1
Protein-Nucleic Acid Interaction and Multiplexing Tutorial
T7 RNA polymerase kinetics and multiplexing of 4 different signals in parallel. Includes 3 reactions.

BUFFERS & SOLUTIONS.

We offer a range of standard buffers and solutions optimized for switchSENSE® experiments.

Edit
Order No. Information
 BU-TE-40-10

10x Buffer TE40 pH 7.4
50 ml of: 100 mM Tris-HCl, 400 mM NaCl, 0.5 % Tween20, 0.5 mM EDTA, 0.5 mM EGTA; Yields 500mL of: 10 mM Tris-HCl, 40 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA

BU-PE-40-10

10x Buffer PE40 pH 7.4
50 ml of: 100 mM Na₂HPO₄/NaH₂PO₄, 400 mM NaCl, 0.5 % Tween20, 0.5 mM EDTA, 0.5 mM EGTA; Yields 500mL of: 10 mM Na₂HPO₄/NaH₂PO₄, 40 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA

BU-HE-40-10

10x Buffer HE40 pH 7.4
50 ml of: 100 mM Hepes, 400 mM NaCl, 0.5 % Tween20, 0.5 mM EDTA, 0.5 mM EGTA; Yields 500mL of: 10 mM Hepes, 40 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA

BU-TE-140-10

10x Buffer TE140 pH 7.4
50 ml of: 100 mM Tris-HCl, 1.4 M NaCl, 0.5 % Tween20, 0.5 mM EDTA, 0.5 mM EGTA; Yields 500mL of: 10 mM Tris-HCl, 140 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA

BU-PE-140-10

10x Buffer PE140 pH 7.4
50 ml of: 100 mM Na₂HPO₄/NaH₂PO₄, 1.4 M NaCl, 0.5 % Tween20, 0.5 mM EDTA,
0.5 mM EGTA; Yields 500mL of: 10 mM Na₂HPO₄/NaH₂PO₄, 140 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA

BU-HE-140-10

10x Buffer HE140 pH 7.4
50 ml of: 100 mM Hepes, 1.4 M NaCl, 0.5 % Tween20, 0.5 mM EDTA, 0.5 mM EGTA; Yields 500mL of: 10 mM Hepes, 140 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA

BU-C-150-1

1x Buffer C pH 8.0
12 ml of: 50 mM Na₂HPO₄/NaH₂PO₄, 150 mM NaCl

BU-P-150-10

10x Buffer A pH 7.2
50 ml of: 500 mM Na₂HPO₄/NaH₂PO₄, 1.5 M NaCl;
Yields 500mL of: 50 mM Na₂HPO₄/NaH₂PO₄, 150 mM NaCl

BU-P-1000-5

5x Buffer B pH 7.2
50 ml of: 250 mM Na₂HPO₄/NaH₂PO₄, 5 M NaCl; Yields 250mL of: 50 mM Na₂HPO₄/NaH₂PO₄, 1 M NaCl

BU-P-5-10

10x P5 Buffer pH 7.3
50 ml of: 50 mM NaH₂PO₄/Na₂HPO₄, pH 7.3; Yields 500mL of: 5 mM NaH₂PO₄/Na₂HPO₄, pH 7.3

BU-M-150-1

1x Buffer MES pH 6.5
12 ml of: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 150 mM NaCl

SOL-PAS-1-5

10x Passivation solution
for passivation of chip surface (5 x 1 ml)

SOL-REG-12-1

Regeneration solution
for regeneration of chip surface (1 x 12 ml)

SOL-REG-UR-12-1

Urea solution
7 M urea (1 x 12 ml)

Consumables.

All the necessary glassware, vials, well plates and other consumables you may need for your switchSENSE® studies.

Edit
Order No. Information
 WP-096-150-10

Microplate 96 well, 280 µl
PP, V-bottom, 10 pcs.

WP-096-650-8

Microplate 96 well, 650µl
PP, V-bottom, 8 pcs.

WP-096-2000-1

Microplate 96 well, 2000 µl
PP, V-bottom, 1 pc.

WP-384-225-6

Microplate 384 well, 200 µl
PP, V-bottom, 6 pcs.

WP-SEAL-HLX

Sealing for 96 and 384 well plates (self-adhesive), 10 pcs.

WP-SEAL-HOT

Sealing for 96 and 384 well plates (only for heat sealing), 10 pcs.

AV-015-100N

Autosampler vials 1.3 ml (glass)
1.3 ml autosampler vials and caps, 100 pcs.

AV-100-100

Autosampler vials 10 ml (glass)
10 ml autosampler vials and caps, 100 pcs.

BC-250-1

Buffer bottle including cap for proFIRE®
Cap with connectors for 250 ml buffer bottle (glass) with GL 45 thread

BC-500-1

Waste bottle including cap for proFIRE®
Bottle including Cap with hole for 500 ml waste bottle (glass) with GL 45 thread

BB-1000-1

Buffer bottle 1000 ml including one connector
1000 ml buffer bottle (plastic) including one capillary connector

BO-1000-3

Buffer bottle 1000 ml including three connectors
1000 ml buffer bottle (plastic) including three capillary connectors

CF-003-5

Centrifugal filter unit 3 kDa
Centrifugal filter unit (3 kDa MWCO), 5 pcs.

CF-010-5

Centrifugal filter unit 10 kDa
Centrifugal filter unit (10 kDa MWCO), 5 pcs.

SY-250-1

Injection syringe
250 µl injection syringe for usage with proFIRE®

TB-CC-1-1

Chromatographic column
Chromatographic column for usage with proFIRE®

VR-013-50-1

Autosampler vial rack
Rack for 50 x 1.3 ml autosampler vials (AV-015-100N)

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