The integrated light microscope and CCD camera allow for the visual control of your cells using snapshots. Viability stains in the red or green range can be used for survival optimization.
There is no need to fix the cells, you can perform RT-IC measurements directly after cell culture with living cells. However, you can also use fixed cells, if preferred.
Yes, your RT-IC sample should be a single-cell suspension.
Yes. You simply have to detach them before and put the cell suspension into the autosampler of the heliXcyto. We recommend buffer without Ca2+ and Mg2+ or addition of EDTA for the handling of adherent cells.
You can use any cell type of your choice for RT-IC measurements. We provide heliXcyto chips with different trap sizes suitable for cells from about 6 to 25 µm diameter. There is no precise size limit for eukaryotic cells, since this is also highly dependent on the...
