New heliXcyto chips have a raw fluorescence below 3 x 105 cps in the measurement channel when performing a chip test (LED = 0.5). Stable adherence of fluorescently labeled analytes to the chip material (glass, gold, polymer) might lead to an increase of this...
The normalization peak should be a sharp injection and ejection of Normalization solution, with a plateau ideally in the same fluorescent count range as the highest measurement data. If the normalization peak injection or ejection is not precise the tube passivation...
