free online tool to visualize binding kinetics data
in rateSCALE & rateMAP plots
smart biophysical analysis
for molecular interaction analysis.
Introducing pure protein-DNA conjugates
proFIRE® is a unique system for protein-DNA conjugate preparation, delivering consistent and superior conjugate quality for your experiments.
Molecular Interaction Analysis
Unrivaled Information Content
- Binding Kinetics
- Binding Affinity
- Protein Diameter
- Conformational Change
- Nuclease & Polymerase Activity
- Bispecific Binders & Avidity
- Melting & Thermodynamics
- Multimers & Aggregation
Biophysical Analysis with Electro-Switchable Biosurfaces
Join us for this recorded webinar presentation to learn more about dynamicBIOSENSORS’ pioneering switchSENSE® sensor platform: an automated, fluorescence based biosensor chip technology that employs electrically actuated DNA nanolevers for biophysical and interaction analysis measurements!
This presentation covers the different measurement modes of our novel switchSENSE® technology, as well as its applications in biophysical analysis.
Paper in Bioconjugate Chemistry
Triazine-Modified 7-Deaza-2′-deoxyadenosines: Better Suited for Bioorthogonal Labeling of DNA by PCR than 2′-Deoxyuridines
22 May 2019
Researchers from the Karlsruhe Institute of Technology attached the bioorthogonally reactive 6-Ethynyl-1,2,4-triazine grou to the 7-position of 7-deaza-2-deoxyadenosine triphosphate and to the 5-position of 2-deoxyuridine triphosphate for subsequent labelling. Both, Primer extension experiments (PEX) and PCR amplification, show that 6-ethynyl-1,2,4-triazine is much better tolerated by the DNA polymerase when attached to the 7-position of 7-deaza-2-deoxyadenosine in comparison to the attachment at the 5-position of 2-deoxyuridine. Real-time kinetic observation of DNA polymerase activity during primer extension using switchSENSE® clearly shows this behavior and imply that bioorthogonal labeling strategies are better suited for 7-deaza-2-adenosines than conventional and available 2-deoxyuridines.
Paper in Nature Communications
08 April 2019
In their latest paper, researchers at the ETH Zürich immobilized increasing consecutive numbers of an RNA tandem motifs on a switchSENSE® chip, which could be recognized by the RNA-binding protein RBFOX1/2 RRM. They found that with increasing number of binding motifs the affinity of RBFOX1/2 RRM increased significantly. This was attributed to a cooperative effect due to multiple protein molecules attaching to the DNA when more than one binding site was available.
In line with this, they could show by using the sizing function of the switchSENSE® technology that with increasing number of binding sites the relative size of the bound protein species increased, confirming the binding of multiple protein monomers.
Dr. Michael Schraeml, Head Protein and Enzyme Technologies
ROCHE DIAGNOSTICS GMBH
Products for High-Performance Analysis
switchSENSE® experiments are performed on reusable multi-electrode, multi-channels biochips.
Fully automated switchSENSE® instruments are 96-well plates compatible and manufactured in Germany.
Including coupling kits, starter packs, training & OQ kits, as well as buffers, solutions and other consumables.