Kinetics – Affinity – Avidity
Molecular Interaction Analysis
Unrivaled Information Content
- Binding Kinetics
- Binding Affinity
- Protein Diameter
- Conformational Change
- Nuclease & Polymerase Activity
- Bispecific Binders & Avidity
- Melting & Thermodynamics
- Multimers & Aggregation
DISCOVER MOLECULAR INTERACTIONS
The dynamicBIOSENSORS team is excited to announce the launch of the heliX® line of biosensor instruments!
heliX® pushes the boundaries of what has been possible in biosensing to help you do more:
- Analysis of binding kinetics and molecular conformations in one measurement.
- Screening of conformational changes de novo by real-time conformation referencing.
- Resolving the fastest kinetics with confidence using advanced microfluidics and 10 ms data collection, and taking advantage of improved signal stability for the characterization of high-affinity binders in long dissociation measurements.
- Two-color single-photon counting fluorescence detection for femto-molar sensitivity and the in-depth analysis of bispecific binders.
- Effortless sensor functionalization and advanced ligand density control with the new Adapter Chip.
heliX® is based on a modular technical design and features an automatic chip-loader in conjunction with a 384 well plate compatible autosampler, allowing you to scale-up throughput to thousands of samples per day.
Paper in PNAS
CMT disease severity correlates with mutation-induced open conformation of histidyl-tRNA synthetase, not aminoacylation loss, in patient cells
In the recent issue of PNAS, researchers from The Scripps Research Institute present their latest groundbreaking findings on the neurodegenerative Charcot-Marie-Tooth (CMT) disease. The study demonstrates that the severity of the CMT disease is closely linked to distinct mutations in the histidyl-tRNA synthetase protein.
Using the switchSENSE® technology, the Scripps researchers could measure the variable mutation-induced conformational expansion of the mutated synthetases in comparison to the wild-type protein. Small-angle X-ray scattering (SAXS) and hydrogen/deuterium (HDX) analyses confirmed the switchSENSE® results. These structural and size measurements enabled the ranking of the different mutations, stating a strong correlation with the severity of the CMT disease.
Paper in Bioconjugate Chemistry
Triazine-Modified 7-Deaza-2′-deoxyadenosines: Better Suited for Bioorthogonal Labeling of DNA by PCR than 2′-Deoxyuridines
Researchers from the Karlsruhe Institute of Technology attached the bioorthogonally reactive 6-Ethynyl-1,2,4-triazine grou to the 7-position of 7-deaza-2-deoxyadenosine triphosphate and to the 5-position of 2-deoxyuridine triphosphate for subsequent labelling. Both, Primer extension experiments (PEX) and PCR amplification, show that 6-ethynyl-1,2,4-triazine is much better tolerated by the DNA polymerase when attached to the 7-position of 7-deaza-2-deoxyadenosine in comparison to the attachment at the 5-position of 2-deoxyuridine. Real-time kinetic observation of DNA polymerase activity during primer extension using switchSENSE® clearly shows this behavior and imply that bioorthogonal labeling strategies are better suited for 7-deaza-2-adenosines than conventional and available 2-deoxyuridines.
Dr. Michael Schraeml, Head Protein and Enzyme Technologies
ROCHE DIAGNOSTICS GMBH
Products for High-Performance Analysis
switchSENSE® experiments are performed on reusable multi-electrode, multi-channels biochips.
Fully automated switchSENSE® instruments are 96-well plates compatible and manufactured in Germany.
Including coupling kits, starter packs, training & OQ kits, as well as buffers, solutions and other consumables.