Kinetics – Affinity – Avidity
Molecular Interaction Analysis
Unrivaled Information Content
- Binding Kinetics
- Binding Affinity
- Protein Diameter
- Conformational Change
- Nuclease & Polymerase Activity
- Bispecific Binders & Avidity
- Melting & Thermodynamics
- Multimers & Aggregation
Biophysical Analysis with Electro-Switchable Biosurfaces
Join us for this recorded webinar presentation to learn more about dynamicBIOSENSORS’ pioneering switchSENSE® sensor platform: an automated, fluorescence based biosensor chip technology that employs electrically actuated DNA nanolevers for biophysical and interaction analysis measurements!
This presentation covers the different measurement modes of our novel switchSENSE® technology, as well as its applications in biophysical analysis.
Paper in PNAS
CMT disease severity correlates with mutation-induced open conformation of histidyl-tRNA synthetase, not aminoacylation loss, in patient cells
09 September 2019
In the recent issue of PNAS, researchers from The Scripps Research Institute present their latest groundbreaking findings on the neurodegenerative Charcot-Marie-Tooth (CMT) disease. The study demonstrates that the severity of the CMT disease is closely linked to distinct mutations in the histidyl-tRNA synthetase protein.
Using the switchSENSE® technology, the Scripps researchers could measure the variable mutation-induced conformational expansion of the mutated synthetases in comparison to the wild-type protein. Small-angle X-ray scattering (SAXS) and hydrogen/deuterium (HDX) analyses confirmed the switchSENSE® results. These structural and size measurements enabled the ranking of the different mutations, stating a strong correlation with the severity of the CMT disease.
Paper in Bioconjugate Chemistry
Triazine-Modified 7-Deaza-2′-deoxyadenosines: Better Suited for Bioorthogonal Labeling of DNA by PCR than 2′-Deoxyuridines
22 May 2019
Researchers from the Karlsruhe Institute of Technology attached the bioorthogonally reactive 6-Ethynyl-1,2,4-triazine grou to the 7-position of 7-deaza-2-deoxyadenosine triphosphate and to the 5-position of 2-deoxyuridine triphosphate for subsequent labelling. Both, Primer extension experiments (PEX) and PCR amplification, show that 6-ethynyl-1,2,4-triazine is much better tolerated by the DNA polymerase when attached to the 7-position of 7-deaza-2-deoxyadenosine in comparison to the attachment at the 5-position of 2-deoxyuridine. Real-time kinetic observation of DNA polymerase activity during primer extension using switchSENSE® clearly shows this behavior and imply that bioorthogonal labeling strategies are better suited for 7-deaza-2-adenosines than conventional and available 2-deoxyuridines.
Dr. Michael Schraeml, Head Protein and Enzyme Technologies
ROCHE DIAGNOSTICS GMBH
Products for High-Performance Analysis
switchSENSE® experiments are performed on reusable multi-electrode, multi-channels biochips.
Fully automated switchSENSE® instruments are 96-well plates compatible and manufactured in Germany.
Including coupling kits, starter packs, training & OQ kits, as well as buffers, solutions and other consumables.