Introducing pure protein-DNA conjugates
proFIRE® is a unique system for protein-DNA conjugate preparation, delivering consistent and superior conjugate quality for your experiments.
smart biophysical analysis
for molecular interaction analysis.
Molecular Interaction Analysis
Unrivaled Information Content
- Binding Kinetics
- Binding Affinity
- Protein Diameter
- Conformational Change
- Nuclease & Polymerase Activity
- Bispecific Binders & Avidity
- Melting & Thermodynamics
- Multimers & Aggregation
Dr. Michael Schraeml, Head Protein and Enzyme Technologies
ROCHE DIAGNOSTICS GMBH
Celastrol Promotes Weight Loss in Diet-Induced Obesity by Inhibiting the Protein Tyrosine Phosphatases PTP1B and TCPTP in the Hypothalamus
Dec. 7th, 2018
Researchers from the Technical University of Munich and the Helmholtz Zentrum München found that the small molecule Celastrol promotes weight loss in obese mice by interaction with the Protein Tyrosine Phosphatases PTP1B.
Using the switchSENSE® technology, they were able to precisely determine the affinity between Celastrol and PTP1B as well as the binding kinetics of this interaction.
Paper in CHEMISTRY 2018
Structural and Kinetic Profiling of Allosteric Modulation of Duplex DNA Induced by DNA-Binding Polyamide Analogues
Nov. 8th, 2018
Together with scientists from the University of Strathclyde we used the switchSENSE® technology to study polyamide molecules that specifically recognize certain DNA sequences by binding to the DNA’s minor grove. Comprehensive kinetic interaction and DNA melting studies provide deep insight into how different chemical modifications of the polyamides alter the binding properties to the DNA. These findings are correlated to data from NMR structure analysis that reveal conformational differences of the DNA duplex depending on the type of chemical modification.
Products for High-Performance Analysis
switchSENSE® experiments are performed on reusable multi-electrode, multi-channels biochips.
Fully automated switchSENSE® instruments are 96-well plates compatible and manufactured in Germany.
Including coupling kits, starter packs, training & OQ kits, as well as buffers, solutions and other consumables.