DISCOVER 
MOLECULAR
INTERACTIONS

Kinetics – Affinity – Avidity
Conformational Changes
Thermodynamics

heliX®

Webinar on July 14, 2020

Antiviral discovery using switchSENSE®:
Advances in polymerase characterization for the COVID-19 era

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switchSENSE® Technology
Molecular Interaction Analysis
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  • Binding Kinetics
  • Binding Affinity
  • Protein Diameter
  • Conformational Change
  • Nuclease & Polymerase Activity
  • Bispecific Binders & Avidity
  • Melting & Thermodynamics
  • Multimers & Aggregation

Application Areas

Introducing heliX®

DISCOVER
MOLECULAR INTERACTIONS.

The dynamicBIOSENSORS team is excited to announce the launch of the heliX® line of biosensor instruments!

NEWS

heliX® pushes the boundaries of what has been possible in biosensing to help you do more:

Analysis of binding kinetics and molecular conformations in one measurement.

Screening of conformational changes de novo by real-time conformation referencing.

Resolving the fastest kinetics with confidence using advanced microfluidics and 10 ms data collection, and taking advantage of improved signal stability for the characterization of high-affinity binders in long dissociation measurements.

Two-color single-photon counting fluorescence detection for femto-molar sensitivity and the in-depth analysis of bispecific binders.

Effortless sensor functionalization and advanced ligand density control with the new Adapter Chip.

heliX® is based on a modular technical design and features an automatic chip-loader in conjunction with a 384 well plate compatible autosampler, allowing you to scale-up throughput to thousands of samples per day.

In focus

Browse through the latest publications.

Paper in Scientific Reports I 09 June 2020

The trimer to monomer transition of Tumor Necrosis Factor-Alpha is a dynamic process that is significantly altered by therapeutic antibodies

In this paper, we demonstrate the power of switchSENSE® for size analysis and antibody binding kinetics. In certain inflammatory diseases, such as rheumatoid arthritis, TNF-α is upregulated and forms bioactive homotrimers that promote inflammation. This bioactivation that was previously reported to occur over hours is seen to occur in only minutes when measured in real-time using switchSENSE’s dynamic measurement mode. The kinetics of TNF-α targeting therapeutic antibodies, including HUMIRA, are also observed, including the long dissociation (measured over 30,000 sec!) of TNF-α from these tight binders. This novel approach also sheds some light on the strong differences between these antibodies that were previously thought to have the same mode of action.

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Paper in PNAS I 31 March 2020

An autoinhibitory intramolecular interaction proof-reads RNA recognition by the essential splicing factor U2AF2

switchSENSE® resolves the binding kinetics of the splicing factor U2AF2 to a target RNA sequence. Hyun-Seo Kang of Michael Sattler’s workgroup at the Technical University of Munich used switchSENSE® to confirm the regulatory function on RNA binding of an inter-domain linker in U2AF2 by measuring the association- and dissociation kinetics of the wildtype protein and of a mutant in which the regulatory linker region had been rendered inactive.

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Paper in Scientific Reports I 29 Jan 2020

Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics

Well-defined antibody-DNA conjugates are a very important tool for many bioanalytical applications, including DNA-nanotechnology or proximity ligation assays (PLA). Researchers from the Helmholtz Center Munich used the proFIRE® technology to purify antibody-DNA conjugates. After carefully optimizing each step of the conjugation method using copper-free click-chemistry, the proFIRE® was used to separate between unconjugated IgG, single and double conjugates and the unconjugated oligo. To demonstrate the functionality of the purified antibody-DNA conjugates, a proximity ligation assay was successfully performed afterwards.

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→ click here for a list of all switchSENSE® publications. 

Dr. Michael Schraeml, Head Protein and Enzyme Technologies
ROCHE DIAGNOSTICS GMBH

Products for High-Performance Analysis

Biochips

switchSENSE® experiments are performed on reusable multi-electrode, multi-channels biochips.

DRX Instruments

Fully automated switchSENSE® instruments are 96-well plates compatible and manufactured in Germany.

Consumables

Including coupling kits, starter packs, training & OQ kits, as well as buffers, solutions and other consumables.

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