pure protein-DNA
conjugates

Imagine an effortless workflow for the preparation, purification and analysis of protein-DNA conjugates.

proFIRE®

Confidence in your reaction products.

Straightforward scientific results

Your workflow to well-defined protein-DNA conjugates. 

our solution

The proFIRE® solution for pure protein-DNA conjugates. 

2-step workflow

The proFIRE® workflow – 2 steps to result. 

unmatched ease-of-use

Tablet-based software, optimized injection port and portable fraction collector. 

intuitive software

proFIRE® software for automatic recognition and quantitation of conjugates. 

reagents

proFIRE® conjugation kits and easy-to-use protocols. 

instrument specifications

proFIRE®  instrument specifications. 

Of the last four big instruments I have bought in the last year, a superresolution microscope, a potentiostat, a fancy gradient maker/fractionator, and the proFIRE®, I would say it is by far the most robust and easy to use.

When I look back over about 15 big lab instruments over the last 20 years, I would say it is one of the top 10% in terms of robustness and ease of use, if it separates the compounds you want to separate (which dynamicBIOSENSORS is great about testing first before you buy).

Prof. Paul W. K. Rothemund

Research Professor, Bioengineering at California Institute of Technology – Pasadena, CA/USA

proFIRE®
Introducing pure protein-DNA conjugates.

Join us for this recorded presentation to learn more about dynamicBIOSENSORS’ unique proFIRE® system.

The proFIRE® 
solution.

For pure protein-DNA conjugates. 

Imagine an effortless workflow for the preparation, purification and analysis of protein-DNA conjugates.

proFIRE® is a unique system for protein-DNA conjugate preparation, delivering consistent and superior conjugate quality for your experiments.

 

Have a look at our proFIRE® flyer.

proFIRE® solution provides you with simply pure protein-DNA conjugates. The unique system delivers consistent and superior conjugate quality for your experiment. 

Conventional methods yield heterogenous mixtures of often ill-defined conjugates.

PROTEIN-DNA CONJUGATION

proFIRE® coupling reagents and ready-to-use protocols for intuitive and simple handling.

PURIFIER

proFIRE® high-performance purification and fraction collection system.

ANALYZER

proFIRE® software for automatic recognition and quantitation of conjugates.

proFIRE® in action.

A resource for scientists. 

AppNote / Conjugation of antibodies to oligonucleotides

A straightforward method to conjugate antibodies to oligonucleotides – preparation, purification and their applications

Antibody oligonucleotide con-jugates are playing a significant role for a wide range of diagnostic and therapeutic applications. Therefore, there is great interest in using pure antibody oligonucleotide conjugates for protein detection by binding to specific protein targets. To sustain antibody functionality, as well as an efficient conjugate yield and a pure conjugate, it is important to conjugate under optimized physiological reaction conditions with no adverse effects on the antibodies. Using copper-free click chemistry and the unique feature of oligonucleotides, which allows easy functionalization, modifying antibodies with oligonucleotides is a straightforward technique.

In this application note, we introduce a conjugation kit for an easy-to-use and covalent conjugation of antibodies to oligonucleotides with high efficiency and best performance in functional test. 

DOWNLOAD APP NOTE

Paper / Single- and double-oligonucleotide antibody conjugates

Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics

Oligonucleotide-conjugated antibodies have gained importance for their use in protein diagnostics. The possibility to transfer the readout signal from the protein to the DNA level with an oligonucleotide-conjugated antibody increased the sensitivity of protein assays by orders of magnitude and enabled new multiplexing strategies. A bottleneck in the generation of larger oligonucleotide-conjugated antibody panels is the low conjugation yield between antibodies and oligonucleotides, as well as the lack of product purification methods. In this study, we combined a non-site-directed antibody conjugation technique using copper-free click chemistry with ion-exchange chromatography to obtain purified single and double oligonucleotide-conjugated antibodies. We optimized the click conjugation reaction of antibodies with oligonucleotides by evaluating crosslinker, reaction temperature, duration, oligonucleotide length, and secondary structure.

As a result, we were able to achieve conjugation yields of 30% at a starting quantity as low as tens of nanograms of antibody, which makes the approach applicable for a wide variety of protein analytical assays. In contrast to previous non-site-directed conjugation methods, we also optimized the conjugation reaction for antibody specificity, confirmed by testing with knockout cell lines. The advantages of using single or double oligonucleotide-conjugated antibodies in regards to signal noise reduction are shown within immunofluorescence, proximity ligation assays, and single cell CITE-seq experiments. 

DOWNLOAD PAPER

AppNote / Conjugation and purification of His-tagged proteins to DNA

Site-specific covalent conjugation and purification of His-tagged proteins to DNA for switchSENSE® applications

In this application note, we introduce a conjugation kit for a straightforward, site-selective and covalent conjugation of His-tagged proteins to DNA with high efficiency using lower amounts of protein. Since they constitute one of the starting points for using the switchSENSE® technology, we developed this conjugation kit. 

DOWNLOAD APP NOTE

Flyer / Sizing made easy

Sizing made easy 

Have you ever thought about functionalizing your DNA-Origami with a protein of your choice? 
Or have you struggled with purifying your antibody-DNA conjugate for your Proxmity Ligation Assay? 

The proFIRE® solution provides an easy and straight-forward workflow to design any protein-DNA conjugate ready for your DNA-Origami application and enables you to use DNA of any length up to 150 nts in your conjugation. 

DOWNLOAD FLYER

Discover pure DNA-protein conjugates.

Let´s discuss your requirements. 

The proFIRE® workflow. 

2 steps to result

Unmatched ease-of-use.

 

proFIRE® tablet based software allows real-time monitoring of your conjugate purification.

proFIRE® optimized injection port geometry 
improves and facilitates sample loading.

proFIRE® portable fraction collector for easy sample handling.

Software designed for intuitive protein-DNA conjugate analysis. 

proFIRE® 
reagents. 

Order No.Product Description
PF-NH2-1proFIRE®  Amine Coupling Kit 1 for proteins (>5 kDa)
sufficient for 5 conjugations
PF-NH2-2-B48proFIRE®  Amine Coupling Kit 2 for His-tagged proteins (>5 kDa)
His-mediated conjugation of proteins to B48; sufficient for 3 conjugations
PF-SH-1proFIRE®  Thiol Coupling Kit 1 for proteins (>5 kDa)
sufficient for 5 conjugations
PF-AB-1proFIRE®  Antibody Oligo Conjugation Kit 1
conjugation of an antibody to an oligonucleotide; sufficient for 3 conjugations
PF-AB-2-B48proFIRE®  Antibody Oligo Conjugation Kit 2
conjugation of an antibody to an oligonucleotide B48; sufficient for 3 conjugations
CF-003-5Centrifugal filter unit (3 kDa MWCO), 5 pcs.
CF-010-5Centrifugal filter unit (10 kDa MWCO), 5 pcs.
PF-CC-1proFIRE®  column
PF-SY-250Injection syringe for proFIRE® 
PF-BU-A-1010 x proFIRE®  Buffer A
PF-BU-B-55 x proFIRE®  Buffer B
BF-BU-C-11 x Conjugation Buffer

proFIRE® 
instrument specifications. 

 

DNA length  |  10 – 150 nt
Protein molecular weight  |  5 – 500 kDa
Injection volume  |  50 – 150 μL
Number of fractions  |  12 
Fraction volume  |  200 – 800 μL
Temperature range  |  10 – 30°C

Discover pure DNA-protein conjugates.

See proFIRE® in action. 

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