Biophysical Analysis of Molecular Interactions
switchSENSE® User Meetings:
Don´t miss out on the latest developments in multi-parameter biophysical analysis of molecular interactions.
Join a variety of scientific talks & participate in technical tutorials in a relaxed atmosphere.
BE INSPIRED, interACT!, ENGAGE & LEARN.
switchSENSE® User Meetings attract leading scientists and speakers from around the world.
interACT! with experts from academia and industry who will be sharing their work in areas from basic research to quality control using switchSENSE® technology.
switchSENSE® User Meetings:
ANTIBODIES & BISPECIFICS
Avidity vs. affinity
Bispecific antibodies form a valuable class of therapeutic agents due to their ability to engage two distinct targets simultaneously. Dual targeting is thought to enhance biological efficacy and increase target selectivity via avidity effect upon binding to both antigens on the surface of the same cell. switchSENSE® biosensor design enables to mimic cell surfaces through the immobilization of two different target antigens on its sensor surface. The precise control over the relative abundance and the spatial arrangement of two target species on the sensor surface is achieved using DNA-guided antigen functionalization and simultaneous dual-color fluorescence detection. The quantitative analysis of individual and cooperative target binding provides invaluable insight, supporting the engineering of the antibody’s individual affinities to achieve minimal off-target binding and maximize selectivity.
DNA / RNA - PROTEIN INTERACTIONS
CRISPR/Cas9, polymerases, transcription factors
Cellular processes such as transcription, translation, or replication require the tightly controlled interaction of proteins with DNA and RNA. The oligonucleotides immobilized on switchSENSE® biosensors are ideally suited as ‘anchors’ to functionalize the sensor surface with DNA or RNA targets of interest, and these targets can be exchanged on the sensor in a straightforward manner through standardized hybridization and washing protocols. Long-ranged fluorescence energy transfer to the supporting gold serves as a molecular ruler to observe movement of proteins along the DNA/RNA molecules in real-time. Hence, enzymatic activity can be gauged.
Quantitation through Stokes radius measurement
Molecular interactions and protein function crucially depend on protein structure and often involve conformational changes. switchSENSE® is a unique platform for the analysis of conformational changes in proteins by combining high sensitivity kinetics with structural analysis. When proteins are bound to the oscillating DNA nanolevers on the switchSENSE® chip, the speed of the electrically actuated nanolevers depends on their hydrodynamic drag and can be converted to the protein’s Stokes diameter with Angstrom resolution. The kinetics of conformational changes or multi-/monomerizations are resolved by monitoring the switching speed in real-time. The conformation signal usually is clearly distinguishable from a pure binding event, and hence binding and structural changes can be detected simultaneously.
Further information will follow soon.
TENTATIVE AGENDA* 2018
|DAY 1 I||12:30pm – 05:00pm||TALKS
DNA/RNA – Protein Interactions
|DAY 2 I||09:00am – 12:30pm||TALKS
Antibodies & Bispecifics
Surface Functionalization & Ligand Density Control
|DAY 3 I||09:00am – 12:30pm||TALKS
Do it Yourself!
*This agenda is subject to change.
CLICK HERE TO DOWNLOAD
Detailed programs of our 2018 user meetings will be available soon.
Join our switchSENSE® tutorials offered during the user meetings in Munich and San Diego at no extra cost
- Measurement modes
- Surface functionalization & ligand density control
- Size analysis
- Data analysis
DO IT YOURSELF!
During our user meetings in Munich and San Diego we also offer you optional LAB COURSES.
Hands-on switchSENSE® experiments with DRX and DRX2 instruments, assisted by DBS scientists.
Samples are provided by Dynamic Biosensors.
Speak at our 2018 User Meetings
Our 2018 switchSENSE® User Meetings are a great opportunity to showcase your research.
Are you interested in giving a presentation? Great to hear!
Abstracts must describe original work and should not be more than 300 words / max. 2.000 characters long.
The deadline for submitting your abstract is May 15th, 2018.
Please fill out the following form to register for the switchSENSE® User Meeting:
till 30 April 2018
- 180 EUR / 220 USD for participants from industry
- 120 EUR / 150 USD for participants from academia
after 30 April 2018
- 220 EUR / 270 USD for participants from industry
- 160 EUR / 200 USD for participants from academia
Please be advised that photographs and/or videos will be taken during our events. By taking part in our user meetings you grant the event organizers full rights to use the images resulting from the photography/video filming, and any reproductions or adaptations of the images for use on the DBS website and in the press, our marketing materials, and other DBS publications.
If you do not wish to be photographed, please inform us in advance.
Thank you for your understanding.
A limited number of rooms are available at the Motel One Munich-City-West at the standard rate of 79,00 € per night/single room and at the Novotel Munich City Arnulfpark at a reduced rate of 130,00 € per night/single room (till 15th of May).
If you´d like to book your room in one of these hotels, please make sure to use the keyword “switchSENSE User Meeting 2018” when contacting the hotel for reservation. Thank you.
WE WILL GET IN TOUCH WITH YOU SHORTLY.
What are you interested in? *
Consumables & Reagents
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New to switchSENSE®
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