No, the heliXcyto does not include an incubator. We recommend using a well buffered solution for your cell suspension, that is not dependent on CO2 buffering. Furthermore, you can adjust the temperature of the sample tray to any temperature between 4 °C – 40...
Both the sample tray (where samples are stored before injection) as well as the chip (where the measurement takes place) are independently temperature-controlled. The chip temperature can be adjusted to 15 °C – 40 °C. The temperature range for the sample tray is 4 °C...
The dissociation script records only the dissociation of your analyte. In a dissociation experiment, the cells are pre-incubated with the labeled analyte before being transferred into the autosampler of the heliXcyto (comparable to immunostainings for FACS or...
Due to optimized fluidics you only need 35 μl of cell suspension per run with a cell concentration of about 1 x 106 cells per ml.
RT-IC can detect low-expression molecules down to a density of about 1000 molecules / cell. The exact detection limit depends on the analyte labeling and affinity.
