The dissociation script records only the dissociation of your analyte. In a dissociation experiment, the cells are pre-incubated with the labeled analyte before being transferred into the autosampler of the heliXcyto (comparable to immunostainings for FACS or microscopy). This method reduces measurement time and sample consumption and guarantees maximum binding signal. It is therefore ideally suited if you aim for higher throughput and want to screen many analytes for their binding capacity and stability. If your research question involves the association rate of your molecule, please choose the kinetic script.
Category: Experimental Considerations