You can analyze any analyte of your choice with RT-IC, from small molecules to cytokines to bispecific antibodies to antigen-MHC complexes. The only prerequisite is that it needs to be fluorescently labelled.
The following parameters can be measured from analytes binding directly to target cells: Association rate kon Dissociation rate koff Affinity, avidity, stability, Kd, t1/2 Fluorescence signal amplitude Fcps, relative target density
Real-Time Interaction Cytometry (RT-IC) is a novel technology applying principles of biophysics and cytometry to enable the measurement of real-time kinetics directly on cells. The unique heliXcyto chip allows for the instantaneous and reversible immobilization of any...
If you double-click on the fit in your analysis, all used mathematical formulas will be displayed.
Ideally, one should start with a scientific hypothesis for the model (e.g. “I expect a basic one-to-one binding process.”). This hypothesis is then tested against the data. If you have no (good) idea about the process, start with the simplest / most confined model and...
