It is advisable to use a detergent-free running buffer (e.g. PE40 without Tween-20). Liposomes can for example be immobilized on the chip surface using a cholesterol-ligand, i.e. cholesterol modified DNA nanolever (cholesterol intercalates into lipid bilayer). Use low...
For kinetic experiments, choosing the appropriate flow rate can be a crucial factor for determining the correct affinity. Insufficient flow rates can potentially lead to measurement artifacts. During the association phase, a too slow flow rate can be the cause for...
Yes, the heliX+ instrument has three different buffer lines. One is used for the maintenance buffer, facilitating standard protocols like functionalization. The other two can be equipped with your individual measurement buffers. The heliOS software has a smart priming...
We know your samples are precious. So we optimized the switchSENSE® technology for minimum sample consumption. You will need approximately 5 pmol ligand per experimental run. Of course, this highly depends on your exact experimental conditions. You can always contact...
The heliX+ instruments were designed to fit your specific needs. You can easily scale up your throughput by combining as many heliX+ modules as you require. This modular design offers high versatility – you can combine heliX units in one heliOS network, operate them...
