It is advisable to use a detergent-free running buffer (e.g. PE40 without Tween-20). Liposomes can for example be immobilized on the chip surface using a cholesterol-ligand, i.e. cholesterol modified DNA nanolever (cholesterol intercalates into lipid bilayer). Use low flow rates for association and dissociation (e.g. < 50 µl/min) to prevent destabilization of membranes.
Category:
Experimental Considerations