It is advisable to use a detergent-free running buffer (e.g. PE40 without Tween-20). Liposomes can be immobilized on the biosurface using a cholesterol-ligand, i.e. cholesterol modified DNA nanolever (cholesterol intercalates into lipid bilayer). Use low flow rates...
As the DNA switching process is extremely sensitive to changes in the hydrodynamic drag, minor changes in the molecular structure of proteins and other macromolecules can be detected as changes in the switching dynamics. A typical application of such an experiment, is...
For kinetic experiments, choosing the appropriate flow rate can be a crucial factor for determining the correct affinity. This can possibly lead to measurement artifacts that are caused by insufficient flow rates. During the association phase, a too slow flow rate can...
The switchBUILD kinetic tool helps to easily set up an optimized taskflow to analyze the interaction of interest and automatically suggests concentration ranges and measurement times based on the estimated affinity of the interaction partners. When no affinity...
There are two basic approaches to detect a conformational change: The first approach is to include a sizing measurement into a kinetic. This way, the association and dissociation kinetics of the test compound to the ligand molecule will be recorded and at equilibrium...
