What should I consider when working with liposomes/vesicles/bilayers?
- It is advisable to use a detergent-free running buffer (e.g. PE40 without Tween-20).
- Liposomes can be immobilized on the biosurface using a cholesterol-ligand, i.e. cholesterol modified DNA nanolever
(cholesterol intercalates into lipid bilayer).
- Use low flow rates for association and dissociation (e.g. < 50 µl/min) to prevent destabilization of membranes.
- Commonly used liposome concentration for sufficient surface coverage: 300 µl of a 0.2 mM solution (phospholipid concentration)