• It is advisable to use a detergent-free running buffer (e.g. PE40 without Tween-20).
  • Liposomes can be immobilized on the biosurface using a cholesterol-ligand, i.e. cholesterol modified DNA nanolever
    (cholesterol intercalates into lipid bilayer).
  • Use low flow rates for association and dissociation (e.g. < 50 µl/min) to prevent destabilization of membranes.
  • Commonly used liposome concentration for sufficient surface coverage: 300 µl of a 0.2 mM solution (phospholipid concentration)
Category: Specific Experimental Considerations