His-tagged proteins conjugation
With dynamicBIOSENSORS´ new site-specific labelling kits His-tagged proteins can be covalently conjugated to DNA in an orientated way with high efficiency.
Well-defined protein-DNA conjugates are a very important tool for many bioanalytical applications.
With our kits, covalent conjugation of proteins to DNA can be carried out in an effortless workflow.
Site-specific covalent conjugation and purification of His-tagged proteins to DNA
A comparison of reaction yields from a conjugation of protein A and a 48mer DNA with (right) and without (left) our proprietary guiding DNA method demonstrates not only more site selective conjugation using this method, but also a 50% higher relative yield.
Figure | Comparison of a reaction mixture of 50 µg protein A with 48mer DNA without addition of Guiding DNA (left) and with addition of Guiding DNA (right).
GET PRACTICAL INSIGHTS
Preparation of pure antibody-DNA conjugates
Join us for this recorded webinar presentation to learn more about dynamicBIOSENSORS’ unique proFIRE® system and get insights into the preparation of pure antibody-DNA conjugates.
„Of the last four big instruments I have bought in the last year,
a superresolution microscope, a potentiostat, a fancy gradient maker/fractionator, and the proFIRE®,
I would say it is by far the most robust and easy to use.
When I look back over about 15 big lab instruments over the last 20 years,
I would say it is one of the top 10% in terms of robustness and ease of use, if it separates the compounds you want to separate (which dynamicBIOSENSORS is great about testing first before you buy).“
Discuss your specific needs with our specialists.