Nucleic Acid Binding Molecules
Study nucleic acid binding molecules with switchSENSE®.
Molecular interactions with nucleic acids
Studying nucleic acid binding molecules with switchSENSE®
Enzymatic Activity and Inhibitor Screening
Nucleic Acids and Small Molecules
Damage Recognition and Repair Mechanisms
Easy biosensor modification with specific nucleic acid sequences
Real-time off-target binding information
High sensitivity up to fM range
Automated assays and data analysis
Determination of cooperativity/avidity effects of complex binders
Characterization of kinetics and catalytic rates of enzymes
Screening of inhibitors (IC50)
Small molecule interactions with nucleic acids
Basic Binding Kinetics /
In this 20 min tutorial, learn how easy it is to measure binding kinetics with our new heliX® biosensors.
We´ll be analyzing both association and dissociation rates as well as Kd-values, using only 8 pmol/run of sample at a cost of just 1.30 EUR per datapoint.
Watch the video to learn how heliX® enables you to characterize molecular interactions in a user friendly, cost effective, and reproducible way.
Nucleotide binding kinetics and conformational change analysis of tissue transglutaminase with switchSENSE®
September 15, 2020 I Analytical Biochemistry
Function, activity, and interactions of proteins crucially depend on their three-dimensional structure and are often regulated by effector binding and environmental changes. Tissue transglutaminase (Transglutaminase 2, TG2) is a multifunctional protein, allosterically regulated by nucleotides and Ca2+ ions, which trigger opposing conformational changes. Here we introduce switchSENSE® as a versatile tool for TG2 characterization and provide novel insights into protein conformation as well as analyte binding kinetics.
For the first time, we succeeded in measuring the kinetic rate constants and affinities (kon, koff, KD) for guanosine nucleotides (GMP, GDP, GTP, GTPγS). Further, the conformational changes induced by GDP, Ca2+ and the covalent inhibitor Z-DON were observed by changes in TG2’s hydrodynamic diameter. We confirmed the well-known compaction by guanosine nucleotides and extension by Ca2+, and provide evidence for TG2 conformations so far not described by structural analysis.
Moreover, we analyze the influence of the peptidic Z-DON inhibitor and the R580A mutation on the conformational responsiveness of TG2 to its natural effectors. In summary, this work shows how the combination of structural and kinetic information obtained by switchSENSE® opens new perspectives for the characterization of conformationally active proteins and their interactions with ligands, e.g. potential drug candidates.
“At Merck, we see the switchSENSE® technology as a versatile and highly valuable tool to positively impact preclinical drug discovery projects. switchSENSE® has proven to be extremely helpful when it comes to the analysis of interactions between a drug candidate and its target protein.
Especially the formation of ternary complexes (DNA-Enzyme-Drug) and being able to detect the inhibition of the enzymatic activity is a big benefit. Moreover, we highly appreciate the support and the competence of the dynamicBIOSENSORS team.”
“switchSENSE® was a revelation: it solved a seemingly intractable antibody/antigen measurement with ease. The key was its remarkably versatile architecture, switchSENSE® seems only limited by the imagination of the researcher.“
“The architectures available from switchSENSE® are uniquely placed to offer us the flexibility and extraordinary sensitivity in our molecular interaction studies. This new technology will immediately open up a number of new research approaches, previously unattainable from existing technologies.“
→ Read more about what our customers say about us.
Find exemplary applications in life science research and drug discovery, for which switchSENSE® provides unique capabilites. Browse through publications, application notes, tutorials, and more.
Measure the kinetics and affinities of DNA/RNA binding proteins directly with switchSENSE®’s unique DNA nanolever approach.
Unravel the behaviour of complex binders like antibodies, PROTACs, and other ternary complexes with switchSENSE®’s versatile binding assays.
Conformation & Size
Solve the structure=function equation and study conformational changes in real-time with switchSENSE®’s dynamic measurement mode.
Discuss your specific needs with our specialists.