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Nanolever temperature stability
standard DNA nanolever I App Note
The oligodeoxynucleotides used as standard nanolevers have been selected to maintain their double stranded conformation even at high temperatures and can be used up to 70°C in T50 buffer and up to 75°C in T20/5 buffer. When performing measurements with proteins at variable temperatures it is crucial that the employed DNA nanolevers remain intact and do not denature.