App Note I January 25, 2014

Nanolever temperature stability
standard DNA nanolever

The oligodeoxynucleotides used as standard nanolevers have been selected to maintain their double stranded conformation even at high temperatures and can be used up to 70°C in T50 buffer and up to 75°C in T20/5 buffer. When performing measurements with proteins at variable temperatures it is crucial that the employed DNA nanolevers remain intact and do not denature.