Real-time measurement of DNA polymerase activity and inhibition with switchSENSE^® and the heliX^® biosensor

Polymerases play a key role in all living cells for the replication and preservation of genetic information. They are also interesting drug targets for several diseases and viral infections, and they find applications in the biotechnology field, such as in PCR and in DNA sequencing. Monitoring the real-time activity of these enzymes not only allows for their characterization but also facilitates inhibition studies and engineering to meet the pharmaceutical demand.
The heliX^® biosensor, featuring a simple workflow and customizable chip, enables straightforward evaluation of enzymatic activity across various enzymes. Herein, we show how to perform real-time measurements of Taq polymerase elongation activity and inhibition.