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Repeatability of Binding Kinetics Measurements and Regeneration Performance of a switchSENSE® Sensor I App Note

The repeatability of binding kinetics measurements and the regeneration performance of a switchSENSE Multi-Purpose Chip (MPC) are presented in this Application Note. Association and dissociation kinetics of a small molecule analyte (desthiobiotin) and a protein ligand (streptavidin) were measured with a DRX instrument. 40 subsequent runs including association and dissociation phases and surface regeneration steps were performed on one measurement spot, i.e., the same microelectrode. The evaluated association and dissociation rate constants kON and kOFF were found to be highly reproducible with a coefficient of variation of less than 3%.

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