App Note I January 7, 2019

Reverse Transcriptase binding and activity assays with switchSENSE®

Reverse transcriptase (RT) is an enzyme found in retroviruses that catalyzes the conversion of single-stranded RNA into double-stranded cDNA. This function of the protein is widely used in the field of molecular biology.

switchSENSE® can be used to investigate the binding and elongation properties of different RTs. Here we analyzed the association of multiple M-MLV RT wild-type concentrations to an RNA template. Subsequently, and as a function of degree of RNA template saturation, the elongation of the DNA primer was measured in real-time.