App Note I March 3, 2018
High-affinity capturing of tagged proteins on the switchSENSE® biochip using Strep-Tactin®XT
Antibody oligonucleotide conjugates are playing a significant role for a wide range of diagnostic and therapeutic applications. Therefore, there is great interest in using pure antibody oligonucleotide conjugates for protein detection by binding to specific protein targets. To sustain antibody functionality, as well as an efficient conjugate yield and a pure conjugate, it is important to conjugate under optimized physiological reaction conditions with no adverse effects on the antibodies. Using copper-free click chemistry and the unique feature of oligonucleotides, which allows easy functionalization, modifying antibodies with oligonucleotides is a straightforward technique.
In this application note, we introduce a conjugation kit for an easy-to-use and covalent conjugation of antibodies to oligonucleotides with high efficiency and best performance in functional test.