Reagents

switchSENSE® Solutions for Research & Development

COUPLING KIT
Selection Wizard

 

 

Demo
& QC Kits

Buffers &
Solutions

Coupling
Kits

Coupling Kits

To link a ligand onto the complementary DNA strand, we offer a range of Coupling Kits. For further information about the binding theory and chip functionalization please check our Application Library. In case you have any questions, please contact info@dynamic-biosensors.com.

Email order@dynamic-biosensors.com for ordering and product information.

Order No.KitDNA lengthProduct Description
CK-NH2-1-B48Amine coupling 148for proteins (>5 kDa); cNL-B48 and NHS modifier,
sufficient for 5 different conjugations
CK-NH2-1-A48Amine coupling 1 48for double color applications
for proteins (>5 kDa); cNL-A48 and NHS modifier,
sufficient for 5 different conjugations
CK-NH2-1-B96Amine coupling 1 96for proteins (>5 kDa); cNL-B96 and NHS modifier,
sufficient for 5 different conjugations
CK-NH2-1-A96Amine coupling 196for double color applications
for proteins (>5 kDa); cNL-A96 and NHS modifier,
sufficient for 5 different conjugations
CK-NH2-2-B48Amine coupling 248for proteins (>5 kDa); cNL-B48 and NHS modifier,
sufficient for 5 different conjugations (spin column purification)
CK-NH2-2-B96Amine coupling 296for proteins (>5 kDa); cNL-B96 and NHS modifier,
sufficient for 5 different conjugations (spin column purification)
PF-NH2-1proFIRE® Amine Coupling Kit 1n/afor proteins (>5 kDa)
sufficient for 5 conjugations
PF-NH2-2proFIRE® Amine Coupling Kit 248for His-tagged proteins (>5 kDa)
sufficient for 3 conjugations
CK-NH2-3-B48Amine coupling 348for small molecules and peptides (< 5 kDa); cNL-B48 and NHS modifier,
sufficient for 5 different conjugations
CK-NH2-5-B96Amine coupling 5 96for bi-functional analytes
two antigens (distance 7 nm) - one nanolever NHS modifier,
sufficient for 5 different conjugations
CK-NH2-6-B96Amine coupling 6 96for bi-functional analytes
two antigens (distance 14 nm) - one nanolever NHS modifier,
sufficient for 5 different conjugations
CK-NH2-7-B48Amine coupling 748for proteins (> 5 kDa) with a low pI (pI < 6)
cNL-B48 and NHS modifier,
sufficient for 5 different conjugations
CK-NH2-7-B96Amine coupling 7 96for proteins (> 5 kDa) with a low pI (pI < 6)
cNL-B96 and NHS modifier,
sufficient for 5 different conjugations
CK-SH-1-B48Thiol coupling 148for proteins (>5 kDa)
cNL-B48 and Maleimide modifier,
sufficient for 5 different conjugations
CK-SH-1-B96Thiol coupling 196for proteins (>5 kDa)
cNL-B96 and Maleimide modifier,
sufficient for 5 different conjugations
CK-SH-2-B48Thiol coupling 248for proteins (>5 kDa)
cNL-B48 and Maleimide modifier,
sufficient for 5 different conjugations (spin column purification)
CK-SH-2-B96Thiol coupling 296for proteins (>5 kDa)
cNL-B96 and Maleimide modifier,
sufficient for 5 different conjugations (spin column purification)
CK-SH-3-B48Thiol coupling 348for small molecules and peptides (< 5 kDa)
cNL-B48 and Maleimide modifier,
sufficient for 5 different conjugations
PF-SH-1proFIRE® Thiol Coupling Kit 1n/afor proteins (>5 kDa)
sufficient for 5 conjugations
CK-CLK-1-B48Click coupling 48cNL-B48 with DBCO modification,
sufficient for 5 different reactions
CK-DG-1-B48Digoxigenin 48cNL-B48 modified with digoxigenin,
sufficient for 20 regenerations
CK-DG-1-B96Digoxigenin 96cNL-B96 modified with digoxigenin,
sufficient for 20 regenerations
CK-TN-1-B48Tris-NTA 48cNL-B48 modified with Tris-NTA,
sufficient for 20 regenerations
CK-TN-1-B96Tris-NTA 96cNL-B96 modified with Tris-NTA,
sufficient for 20 regenerations
CK-SA-1-B48Streptavidin 48cNL-B48 modified with Streptavidin,
sufficient for 20 regenerations
CK-SA-1-B96Streptavidin 96cNL-B96 modified with Streptavidin,
sufficient for 20 regenerations
CK-SXT-1-B96Strep-Tactin® XT96cNL-B96 modified with Strep-Tactin® XT*, sufficient for 20 regenerations
CK-CS1-B96 CaptureSelect™ Anti-LC-kappa (Murine) Conjugate96cNL-B96 modified with CaptureSelect™** Anti-LC-kappa (Murine), sufficient for 20 regenerations
CK-CS2-B96CaptureSelect™ Anti-IgG-Fc Rabbit Conjugate96cNL-B96 modified with CaptureSelect™** Anti-IgG-Fc Rabbit, sufficient for 20 regenerations
CK-FC-1-B48Fc binding Protein A48cNL-B48 modified with Protein A,
sufficient for 20 regenerations
CK-FC-1-B96Fc binding Protein A 96cNL-B96 modified with Protein A,
sufficient for 20 regenerations
CK-FC-2-B48Fc binding Protein G 48cNL-B48 modified with Protein G,
sufficient for 20 regenerations
CK-FC-2-B96Fc binding Protein G 96cNL-B96 modified with Protein G,
sufficient for 20 regenerations
CK-GFP-1-B48GFP binding protein 48cNL-B48 modified with GFP binding protein***,
sufficient for 20 regenerations
CK-GFP-1-B96GFP binding protein 96cNL-B96 modified with GFP binding protein***,
sufficient for 20 regenerations
CK-GST-1-B48GST binding protein 48cNL-B48 modified with GST binding protein***,
sufficient for 20 regenerations
CK-GST-1-B96GST binding protein 96cNL-B96 modified with GST binding protein***,
sufficient for 20 regenerations
RK-PA-1Reducing Agent 1 for Proteins and Antibodies
Chemical Reduction of disulfide bonds with TCEP

*Strep-Tactin® XT is a product of IBA GmbH

**Capture SelectTM  is a product of Thermo Fisher Scientific

***GFP / GST binding protein is a product of ChromoTek GmbH

QUESTIONS?

Contact us – we are happy to help.

Demo & QC Kits

Demo & QC Kits are ideal for teaching or revision. Supplied with a detailed user’s manual and reagents they will guide users through a full experiment.

In general we recommend an annual service visit, which includes a complete documented re-qualification of the instrument according to QC standards.

Email order@dynamic-biosensors.com for ordering and product information.

Order No.KitApplication
DK-DC-1-B48Demo kit protein –
small molecule –
Dual color
(kon | koff)
Association and dissociation of two antibody fabs in parallel.
DK-BP-1-B48Demo kit protein –
small molecule
(kon | DH | koff)
Association, size and dissociation of an anti-Biotin Fab fragment, needs Multi-purpose Biochips with NL-B48.
DK-QC-A48-1QC DRX kit
DNA hypridization kinetics using a 7mer oligo
This kit contains all reagents and chips for checking functionality and performance of DRX and DRX2 instruments.

Would you like to get more INFO
or arrange a DEMO?

Contact us, we will get in touch with you shortly!

Buffers & Solutions

We offer a range of standard buffers and solutions optimized for switchSENSE® experiments.

The platform is highly tolerant to a wide range of buffers. Download our compatibility chart at our Application Library.

Email order@dynamic-biosensors.com for ordering and product information.

Order No.Buffer / SolutionProduct description
BU-TE-40-1010x Buffer TE40 pH 7.450 mL of: 100 mM Tris-HCl, 400 mM NaCl, 0.5 % Tween20, 0.5 mM EDTA,0.5 mM EGTA;
Yields 0.5 L of: 10 mM Tris-HCl, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA,50 μM EGTA
BU-PE-40-1010x Buffer PE40 pH 7.450 mL of: 100 mM Na2HPO4/NaH2PO4, 400 mM NaCl, 0.5 % Tween20, 0.5 mM EDTA, 0.5 mM EGTA;
Yields 0.5 L of: 10 mM Na2HPO4/NaH2PO4, 40 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA
BU-HE-40-1010x Buffer HE40 pH 7.450 mL of: 100 mM Hepes, 400 mM NaCl, 0.5 % Tween20, 0.5 mM EDTA, 0.5 mM EGTA;
Yields 0.5 L of: 10 mM Hepes, 40 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA
BU-TE-140-1010x Buffer TE140 pH 7.450 mL of: 100 mM Tris-HCl, 1.4 M NaCl, 0.5 % Tween20, 0.5 mM EDTA, 0.5 mM EGTA;
Yields 0.5 L of: 10 mM Tris-HCl, 140 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA
BU-PE-140-1010x Buffer PE140 pH 7.450 mL of: 100 mM Na2HPO4/NaH2PO4, 1.4 M NaCl, 0.5 % Tween20, 0.5 mM EDTA, 0.5 mM EGTA;
Yields 0.5 L of: 10 mM Na2HPO4/NaH2PO4, 140 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA
BU-HE-140-1010x Buffer HE140 pH 7.450 mL of: 100 mM Hepes, 1.4 M NaCl, 0.5 % Tween20, 0.5 mM EDTA. 0.5 mM EGTA;
Yields 0.5 L of: 10 mM Hepes, 140 mM NaCl, 0.05 % Tween20, 50 µM EDTA, 50 µM EGTA
BU-C-150-11x Buffer C pH 8.050 mL of: 50 mM Na2HPO4/NaH2PO4, 150 mM NaCl
BU-P-150-1010x Buffer A pH 7.250 mL of: 500 mM Na2HPO4/NaH2PO4, 1.5 M NaCl;
Yields 0.5 L of: 50 mM NaH2PO4/NaH2PO4, 150 mM NaCl
BU-P-1000-55x Buffer B pH 7.250 mL of: 250 mM Na2HPO4/NaH2PO4, 5 M NaCl;
Yields 0.25 L of: 50 mM NaH2PO4/NaH2PO4, 1 M NaCl
BU-P-5-1010x P5 Buffer pH 7.350 mL of: 50 mM NaH2PO4/Na2HPO4 pH 7.3;
Yields 0.5 L of: 5 mM NaH2PO4/Na2HPO4 pH 7.3
BU-M-150-11x Buffer MES pH 6.512 mL of: 50 mM 2-(N-Morpholino)ethanesulfonic acid, 150 mM NaCl)
CNL-A48-500cNL-A48 solutionsolution of complementary DNA lever for sequence A48, cNL-A48 500 μL / 1 μM
CNL-A96-500cNL-A96 solutionsolution of complementary DNA lever for sequence A96, cNL-A96 500 μL / 1 μM
CNL-B48-500cNL-B48 solutionsolution of complementary DNA lever for sequence B48, cNL-B48 500 μL / 1 μM
CNL-B96-500cNL-B96 solutionsolution of complementary DNA lever for sequence B96, cNL-B96 500 μL / 1 μM
CNL-DP36-500Solution of dideoxy-P36 DNAsolution of Dideoxy-P36 DNA for usage with ENZ-80-1-R1-X or ENZ-80-1-Y1-X, DDO-P36 500 μL / 1 μM
CNL-P36T80-500P36T80 solutionsolution of P36T80 DNA strand for usage with ENZ-80-1-R1-X or ENZ-80-1-Y1-X, P36T80 500 μL / 1 μM
CNL-P48T80-500P48T80 solutionsolution of P48T80 DNA strand for usage with ENZ-80-1-R1-X or ENZ-80-1-Y1-X, P48T80 500 μL / 1 μM
CNL-P80T80-500P80T80 solutionsolution of P80T80 DNA strand for usage with ENZ-80-1-R1-X or ENZ-80-1-Y1-X, P80T80 500 μL / 1 μM
CNL-QRF-B48-500cNL-B48-QRF solutionsolution of complementary DNA lever for sequence B48 modified with a quenching molecule for the red fluorophore 500 μL / 1 μM
CNL-QRF-B96-500cNL-B96-QRF solutionsolution of complementary DNA lever for sequence B96 modified with a quenching molecule for the red fluorophore 500 μL / 1 μM
CNL-QYF-B48-500cNL-B48-QYF solutionsolution of complementary DNA lever for sequence B48 modified with a quenching molecule for the yellow fluorophore 500 μL / 1 μM
CNL-QYF-B96-500cNL-B96-QYF solutionsolution of complementary DNA lever for sequence B96 modified with a quenching molecule for the yellow fluorophore 500 μL / 1 μM
CNL-QGF-B48-500cNL-B48-QGF solutionsolution of complementary DNA lever for sequence B48 modified with a quenching molecule for the green fluorophore 500 μL / 1 μM
CNL-QGF-B96-500cNL-B96-QGF solutionsolution of complementary DNA lever for sequence B96 modified with a quenching molecule for the green fluorophore 500 μL / 1 μM
CNL-QGF-A48-500QGF solution for green fluorophoresolution of complementary DNA lever for sequence A48 modified with a green fluorophore quencher molecule, cNL-A48-QGF 500 μL / 1 μM
CNL-QGF-A96-500QGF solution for green fluorophoresolution of complementary DNA lever for sequence A96 modified with a green fluorophore quencher molecule, cNL-A96-QGF 500 μL / 1 μM
CNL-Cas-A48-500cNL-Cas-A48 solutionsolution of complementary DNA lever for sequence A48, without PAM-region
cNL-Cas-A48 500 μL / 1 μM
CNL-Cas-B48-500cNL-Cas-B48 solutionsolution of complementary DNA lever for sequence B48, without PAM-region
cNL-Cas-B48 500 μL / 1 μM
SOL-PAS-1-510x Passivation solutionfor passivation of chip surface (5 x 1 mL)
SOL-REG-1-5Regeneration solutionfor regeneration of chip surface (5 x 1mL)
SOL-REG-5-1Regeneration solutionfor regeneration of chip surface (5 x 1 mL)
SOL-REG-UR-12-1Urea solution7 M urea (1 x 12 mL)

Would you like to get more INFO
or arrange a DEMO?

Contact us, we will get in touch with you shortly!

Selection of Coupling Kits

This guide helps you towards the right approach for a successful switchSENSE®experiment.
Please follow the 2-step guide below to be directly transferred to the coupling kit, which is of interest for you.

Step 1 I Immobilization Methods

The biochip surface is functionalized with single-stranded DNA. The versatility of the experiment lies in ability to hybridize a wide-array of complementary DNA sequences. Your molecule of interest, referred to as the ligand, is immobilized onto the double-stranded DNA nanolever, either by direct covalent coupling or by tag-capturing methods.

switchSENSE® offers plenty of solutions for the immobilization of your ligand.
Please choose from the given options on the right.

_

Covalent Coupling

Tag Capture Methods

Step 2 I Covalent Coupling

We offer three different solutions using pre-established protocols for the covalent immobilisation of your biomolecules.

Amine Coupling, Thiol Coupling and Customized Strategies.

Most of them are known for a long time and are easy to use and work under physiological conditions with different kind of buffers and additives (please check in the respective user manual). The kits are optimized to ensure a 1:1 coupling between the nanolevers and ligands.

To ensure highest quality of your conjugate we offer two different purification methods:
1. Chromatographic method for highest performance
2. Spin columns protocol (faster)

Amine Coupling

Thiol Coupling

Customized Strategies

Covalent Coupling Kits

Amine Coupling

For Amine Coupling there are different kits available to address your needs.
You can order kits for the functionalization of 48mer, 96mer and bispecific nanolevers.

In switchSENSE® Amine Coupling Kits we solely use standard pre-established chemistry.

Amine Coupling Kit 1

For proteins (>5 kDa); cNL B48 or B96 and NHS modifier, sufficient for 5 conjugation series.

CK-NH2-1-A48

CK-NH2-1-A96

CK-NH2-1-B48

CK-NH2-1-B96

Amine Coupling Kit 2

For proteins (>5 kDa); cNL B48 or B96 and NHS modifier, sufficient for 5 conjugation series (spin column purification).

CK-NH2-2-B48

CK-NH2-2-B96

Amine Coupling Kit 3

For small molecules and peptides (<5 kDa); cNL-B48 or cNL-B96 and NHS modifier, sufficient for 5 conjugation series.

CK-NH2-3-B48

Amine Coupling Kit 4

For proteins (>5 kDa); cNL B48 or B96 and NHS modifier, sufficient for 5 conjugation series.

CK-NH2-4-B48

Amine Coupling Kit 5

For proteins (>5 kDa); cNL B48 or B96 and NHS modifier, sufficient for 5 conjugation series (spin column purification).

CK-NH2-5-B96

Amine Coupling Kit 6

For small molecules and peptides (<5 kDa); cNL-B48 or cNL-B96 and NHS modifier, sufficient for 5 conjugation series.

CK-NH2-6-B96

Amine Coupling Kit 7

Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube.
Includes reagents for five individual conjugation reactions (approx. 10-50 regenerations each; up to 500).

CK-NH2-7-B48

CK-NH2-7-B96

Thiol Coupling

For Thiol Coupling there are different kits available to address your needs.
You can order kits for the coupling of a ligand to 48mer and 96mer nanolevers.

In switchSENSE® Thiol Coupling Kits we solely use standard maleimide chemistry.

Thiol Coupling Kit 1

For proteins (>5 kDa); cNL B48 or B96 and NHS modifier, sufficient for 5 conjugation series.

CK-SH-1-B48

CK-SH-1-B96

Thiol Coupling Kit 2

For proteins (>5 kDa); cNL B48 or B96 and NHS modifier, sufficient for 5 conjugation series (spin column purification).

CK-SH-2-B48

Thiol Coupling Kit 3

For small molecules and peptides (<5 kDa); cNL-B48 or cNL-B96 and NHS modifier, sufficient for 5 conjugation series.

CK-SH-3-B48

Customized Strategies

Both copper catalyzed and copper-free click chemistry are compatible with the switchSENSE® technology.
For further information on customized solutions, please send an email to support@dynamic-biosensors.com 

Click Coupling Kit

For proteins (>5 kDa); cNL B48 or B96 and NHS modifier, sufficient for 5 conjugation series.

CK-CLK-1-B48

Step 2 I Tag Capture Methods

We offer a large array of options for the non-covalent immobilisation of your biomolecules using tag-capture.

Proteins are expressed with a variety of different tags, which can be targeted for binding to the DNA nanolevers. Complementary DNA conjugated with the second interaction component is hybridized onto the biosensor surface, and used for the capture of your molecule or interest.

Tris-NTA Kit

cNL-B48 and cNL-B96 modified with Tris-NTA, sufficient for 20 regenerations. Suitable for His-tagged proteins.

CK-TN-1-B48

CK-TN-1-B96

Streptavidin Kit

cNL-B48 and cNL-B96 modified with Streptavidin, sufficient for 10 regenerations. Very strong binder of biotinylated molecules.

CK-SA-1-B48

CK-SA-1-B96

FC A Kit

cNL-B48 and cNL-B96 modified with Protein A, sufficient for 10 regenerations. Can be used for capturing antibodies.

CK-FC-1-B48

CK-FC-1-B96

FC G Kit

cNL-B48 and cNL-B96 modified with Protein G, sufficient for 10 regenerations. Can be used for capturing antibodies.

CK-FC-2-B48

CK-FC-2-B96

GFP Binding Protein Kit

cNL-B48 and cNL-96 modified with GFP binding protein*, sufficient for 10 regenerations. Strong binder of GFP-fusion proteins.

CK-GFP-1-B48

CK-GFP-1-B96

GST Binding Protein Kit

cNL-B48 and cNL-B96 modified with GST binding protein*, sufficient for 20 regenerations. Strong binder of GST-fusion proteins.

CK-GST-1-B48

CK-GST-1-B96

Digoxigenin Kit

cNL-B48 and cNL-B96 modified with digoxigenin, sufficient for 20 regenerations.

CK-DG-1-B48

CK-DG-1-B96

Strep-Tactin® XT Kit

cNL-B96 modified with Strep-Tactin® XT, sufficient for 20 regenerations. Strong binder of protein modified with a Twin Strep-tag®.

CK-SXT-1-B96

Selected Kit

CK-NH2-1-A48

Key Features
  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence A48
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 10-50 regenerations each; up to 500)
  • Compatible with automated standard regeneration process
  • Only use in combination with CK-NH2-1-B48 for double color application

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-NH2-1-A96

Key Features

  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Longer nanolever for larger complexes
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence A96
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 10-30 regenerations each; up to 200)
  • Compatible with automated Standard regeneration process
  • Only use in combination with CK-NH2-1-B96 for double color application

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-NH2-1-B48

Key Features
  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B48
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 10-50 regenerations each; up to 500)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-NH2-1-B96

Key Features
  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Longer nanolever for larger complexes
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B96
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 10-30 regenerations each; up to 200)
  • Compatible with automated Standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-NH2-2-B48

Key Features
  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • No chromatographic purification necessary
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B48
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 10-50 regenerations each; up to 500)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-NH2-2-B96

Key Features
  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Longer nanolever for larger complexes
  • No chromatographic purification necessary
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B96
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 10-30 regenerations each; up to 200)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-NH2-3-B48

Key Features
  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for peptides and small molecules (MW < 5 kDa)
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B48
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 200 regenerations each)
  • Compatible with automated Standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-NH2-4-B48

Key Features
  • In comparison with the Standard Amine Coupling Kit 1 this microKit requires only 20% of the starting quantity of protein
  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B48
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 2-20 regenerations each)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-NH2-5-B96

Key Features
  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B96
  • Two ligands – one nanolever
  • Short distance between ligands: 7 nm
  • Suitable for bifunctional molecules, e.g. bispecific antibodies
  • Coupling of two ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate 
  • Includes reagents for 2 x 5 individual conjugation reactions (approx. 10-15 regenerations each; up to 500)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-NH2-6-B96

Key Features
  • Coupling of two biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B96
  • Two ligands – one nanolever
  • Long distance between ligands: 14 nm
  • Suitable for bifunctional molecules, e.g. bispecific antibodies
  • Coupling of two ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate 
  • Includes reagents for 2 x 5 individual conjugation reactions (approx. 10-50 regenerations each; up to 500)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-SH-1-B48

Key Features
  • Coupling of biomolecules with free thiols (e.g. cysteines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B48
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 10-50 regenerations each; up to 500)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-SH-1-B96

Key Features
  • Coupling of biomolecules with free thiols (e.g. cysteines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Longer nanolevers for larger complexes
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B96
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 10-30 regenerations each; up to 200)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-SH-2-B48

Key Features
  • Coupling of biomolecules with free thiols (e.g. cysteines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • No chromatographic purification nesssary
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B48
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate
  • Includes reagents for five individual conjugation reactions (approx. 10-30 regenerations each; up to 200)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-SH-3-B48

Key Features
  • Coupling of biomolecules with free thiols (e.g. cysteines) to modified nanolevers in a reaction tube
  • Convenient standard chemistry
  • Applicable for peptides and small molecules (MW > 5 kDa)
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B48
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate
  • Includes reagents for five individual conjugation reactions (approx. 200 regenerations each)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-CLK-1-B48

Key Features
  • cNL-B48 modified with Dibenzocyclooctyne (DBCO)
  • Coupling of biomolecules with an azide to DBCO modified nanolevers in a reaction tube
  • Bioorthogonal conjugation method
  • Applicable for proteins (and peptides)
    (MW > 5 kDa)
  • Compatible with all switchSENSE® multi-purpose biochips carrying sequence B48
  • Suitable for parallel measurements via DNA encoded addressing
  • Coupling of multiple ligands can be performed simultaneously
  • Yields >95 % pure ligand – DNA conjugate with controlled quality of your product
  • Includes reagents for five individual conjugation reactions (approx. 10-50 regenerations each; up to 500)
  • Compatible with automated standard regeneration process

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-TN-1-B48

Key Features
  • cNL-B48 modified with a Tris-NTA
  • Used for capturing of His-tagged peptides and proteins (KD ~ 5 nM)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B48
  • Suitable for kinetic and titration measurements
  • Suitable for sizing and conformational change analysis
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 x 10 immobilizations of His-tagged peptides and proteins

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-TN-1-B96

Key Features
  • cNL-B96 modified with a Tris-NTA
  • Used for capturing of His-tagged peptides and proteins (KD ~ 5 nM)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B96
  • Suitable for kinetic and titration measurements
  • Suitable for conformational change analysis
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 x 10 immobilizations of His-tagged peptides and proteins

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-SA-1-B48

Key Features
  • cNL-B48 modified with Streptavidin
  • This kit is intendend for use as an immobilization method for biotinylated ligands (KD ~ fM)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B48
  • Suitable for kinetic and titration measurements
  • Suitable for conformational change analysis
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of biotinylated ligands

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-SA-1-B96

Key Features
  • cNL-B96 modified with Streptavidin
  • This Streptavidin-kit is intendend for use as an immobilization method for biotinylated ligands (KD ~ fM)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B96
  • Suitable for kinetic and titration measurements
  • Suitable for conformational change analysis
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of biotinylated ligands

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-SXT-1-B96

Key Features
  • cNL-B96 modified with Strep-Tactin®XT
  • This kit is intendend for use as an immobilization method for Twin-Strep-tag® proteins (KD ~ pM)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B96
  • Suitable for kinetic and titration measurements
  • Suitable for conformational change analysis
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of ligands modified with a Twin-Strep-tag®

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

Strep-Tactin® XT is a product of IBA GmbH

CK-FC-1-B48

Key Features
  • cNL-B48 modified with Protein A
  • This kit is intendend for use as a capture method for various IgG species (KD ~ nM, depending on IgG species)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B48
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of an IgG

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-FC-1-B96

Key Features
  • cNL-B96 modified with Protein A
  • This kit is intendend for use as a capture method for various IgG species (KD ~ nM, depending on IgG species)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B96
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of an IgG

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-FC-2-B48

Key Features
  • cNL-B48 modified with Protein G
  • This kit is intendend for use as a capture method for various IgG species (KD ~ nM, depending on IgG species)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B48
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of an IgG

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-FC-2-B96

Key Features
  • cNL-B96 modified with Protein G
  • This kit is intendend for use as a capture method for various IgG species (KD ~ nM, depending on IgG species)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B96
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of an IgG

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-GFP-1-B48

Key Features
  • cNL-B48 modified with a GFP binding protein
  • This kit is intendend for use as a capture method for GFP-fusion proteins (KD ~ pM)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B48
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of a GFP-fusion protein

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

GFP / GST binding protein is a product of ChromoTek GmbH

CK-GFP-1-B96

Key Features
  • cNL-B96 modified with a GFP binding protein
  • This kit is intendend for use as a capture method for GFP-fusion proteins (KD ~ pM)
  • This kit is intendend for use as a capture method for various IgG species with nM affinity
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B96
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of a GFP-fusion protein

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

GFP / GST binding protein is a product of ChromoTek GmbH

CK-GST-1-B48

Key Features
  • cNL-B48 modified with a GST binding protein
  • This kit is intendend for use as a capture method for GST-fusion proteins (KD ~ pM)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B48
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of a GST-fusion protein

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

GFP / GST binding protein is a product of ChromoTek GmbH

CK-GST-1-B96

Key Features
  • cNL-B96 modified with a GST binding protein
  • This kit is intendend for use as a capture method for GST-fusion proteins (KD ~ pM)
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B96
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of a GST-fusion protein

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

GFP / GST binding protein is a product of ChromoTek GmbH

CK-BI-1-B48

Key Features
  • cNL-B48 modified with Biotin
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B48
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of Streptavidin

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-BI-1-B96

Key Features
  • cNL-B96 modified with Biotin
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B96
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of Streptavidin

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-DG-1-B48

Key Features
  • cNL-B48 modified with Digoxigenin
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B48
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of a Digoxigenin binding protein

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-DG-1-B96

Key Features
  • cNL-B96 modified with Digoxigenin
  • Compatible with all switchSENSE® Multi-purpose biochips carrying sequence B96
  • Suitable for kinetic and titration measurements
  • Suitable for parallel measurements via DNA encoded addressing
  • Includes reagents for 20 immobilizations of a Digoxigenin binding protein

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

CK-NH2-7-B48

Key Features
  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Suitable for parallel measurements via DNA encoded addressing

Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.

 

CK-NH2-7-B96

Key Features
  • Coupling of biomolecules with primary amines (e.g. NH2-terminus, lysines) to modified nanolevers in a reaction tube
  • Suitable for parallel measurements via DNA encoded addressing

 Find out what biochips this kit is compatible with.
DOWNLOAD our coupling kit / biochips compatibility matrix.