If you double-click on the fit in your analysis, all used mathematical formulas will be displayed.
Ideally, one should start with a scientific hypothesis for the model (e.g. “I expect a basic one-to-one binding process.”). This hypothesis is then tested against the data. If you have no (good) idea about the process, start with the simplest / most confined model and...
The heliX® device is equipped with two spots allowing for simultaneous measurements. One of the two spots may be used for the actual binding kinetics experiment (“measurement spot”) while the other one serves as a ligand-free reference (“reference spot”). In this...
The purpose of reference-correction, or referencing for short, is to extract the actual binding kinetics signals from the raw fluorescence signals measured by the heliX® device. Referencing aims to remove any artifacts / side effects such as signal drifts, signal...
The heliX+ instrument enables you to switch between different plate formats and sample vial volumes. You simply need to change the format in heliOS: click on the “sample tray” icon in your assay workflow and choose the format in the dropdown menu.
