RT-IC measurements can be run in any media or buffer of your choice, just make sure to avoid pH indicator dyes such as phenol red. In order to ensure good cell viability throughout the assay it is important to keep them at a physiological pH at all times. Since the heliXcyto does not include an incubator, we recommend using a well buffered solution (e.g. PBS based) for your cell suspension that is not dependent on CO2 for maintaining a physiological pH.
Category: Experimental Considerations