Ensure that your cell suspension is monodisperse (no clumping) and has a concentration of 1 x 105 cells/mL or higher. Resuspend the cell suspension freshly before inserting the samples into the heliXcyto biosensor. You can filter the cell suspension through a cell strainer to reduce clumping. Especially when working with small cells, increasing the cell concentration up to 10 x 105 cells/ml may enhance the capture efficiency.

Category: Troubleshooting