Ensure that your cell suspension is monodisperse (no clumping) and has a concentration of 1 x 106 cells/mL or higher. Resuspend the cell suspension freshly before inserting the samples into the heliXcyto biosensor. You can filter the cell suspension through a cell strainer to reduce clumping. Especially when working with small cells, increasing the cell concentration up to 10 x 106 cells/mL may enhance the capture efficiency.
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