The Lollipop model is a biophysical model used in DRX instruments to provide an estimated size of the protein of interest in form of the hydrodynamic diameter. The model assumes that the DNA nanolevers are a charged and rigid cylinder and the attached protein molecules are a spherical body on top of the cylinder. Data obtained in a dynamic mode measurement are compared with a library of simulated data. A detailed mathematical description is published and can be found here. The application of the lollipop model for protein sizing is limited since it highly depends on e.g. the buffer and the nanolever size. That is why we do not apply it in our new heliX® product line.
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