Molecular Interaction Analysis
Unrivaled Information Content
- Binding Kinetics
- Binding Affinity
- Protein Diameter
- Conformational Change
- Nuclease & Polymerase Activity
- Bispecific Binders & Avidity
- Melting & Thermodynamics
- Multimers & Aggregation
Fresh information, earlier in the pipeline switchSENSE® identifies and thoroughly characterizes potential hits through advanced kinetics and biophysical characterization of size, shape and conformation.
Characterizing enzyme activity and reproducible lot-by-lot quality measurements, switchSENSE® offers a brand new tool – extremely rapid, highly insightful, radiation free.
Paper in Nature Communication 2017 I Specialized interfaces of Smc5/6 control hinge stability and DNA association
Cohesin, condensin and Smc5/6 belong to the group of Structural Maintenance of Chromosomes (SMC) complexes which are involved in the organization of higher-order chromosome structure. The paper exhibits that the Smc5/6-hinge, like those of cohesin and condensin, also forms a toroidal structure, however, with an unusual ‘molecular latch’ and a functional ‘hub’. Defined mutations in these interfaces cause severe phenotypic effects with sensitivity to DNA-damaging agents in fission yeast and reduced viability in human cells. By the switchSENSE® technology it was shown that WT Smc5/6-hinge readily associated with and dissociated from immobilized ssDNA. This interaction is affected by both ‘latch’ and ‘hub’ mutations, suggesting a key role for these unique features in controlling DNA association by the Smc5/6 complex.
Products for High-Performance Analysis
switchSENSE® experiments are performed on reusable multi-electrode, multi-channels biochips.
Fully automated switchSENSE® instruments are 96 well plates compatible and manufactured in Germany.
Including coupling kits, starter packs, training & OQ kits, as well as buffers, solutions and other consumables.
Dr. Michael Schraeml, Head Protein and Enzyme Technologies
ROCHE DIAGNOSTICS GMBH