Generally, switchSENSE® can tolerate measurements in a variety of complex matrices, including cell lysates and high serum concentrations up to 80 %. Nevertheless, measurements in complex media usually require a certain degree of assay development. In most cases, this...
You can separately adjust the chip tray temperature (i.e. the temperature of your measurement) and the sample tray temperature. This allows you for example to keep your samples cold, but measure at higher temperatures. The chip tray temperature can be adjusted to 15...
Yes, you can. And there’s more to it than that. You can even differentiate between the association of DNA/RNA binding enzymes and their catalytic activity and independently examine the effects of inhibitors on these processes.
It is advisable to use a detergent-free running buffer (e.g. PE40 without Tween-20). Liposomes can for example be immobilized on the chip surface using a cholesterol-ligand, i.e. cholesterol modified DNA nanolever (cholesterol intercalates into lipid bilayer). Use low...
For kinetic experiments, choosing the appropriate flow rate can be a crucial factor for determining the correct affinity. Insufficient flow rates can potentially lead to measurement artifacts. During the association phase, a too slow flow rate can be the cause for...
