Generally, switchSENSE® can tolerate measurements in a variety of complex matrices, including cell lysates and high serum concentrations up to 80%. Notably, the robust fluidic system, which DRX instruments are equipped with, allows to handle rather complex solutions....
switchSENSE® measurements can be performed from 8 to 70°C at constant temperatures. Additionally, the temperature of the biochip can be ramped up or down (1-10°C/min), e.g. during a melting experiment. Be aware that the chip life time may suffer from long experiments...
As it is based on surface-immobilized DNA nanolevers, the switchSENSE® technology is particularly suitable to investigate interactions between proteins and nucleic acids. There are two basic experimental setups that are commonly used to investigate protein/nucleic...
In some cases, experiments with peptides can be challenging due to the chemical properties of the individual peptides. Major aspects that can negatively affect your experiments and suggested solutions for troubleshooting are listed below: Peptide solubility: A...
It is advisable to use a detergent-free running buffer (e.g. PE40 without Tween-20). Liposomes can be immobilized on the biosurface using a cholesterol-ligand, i.e. cholesterol modified DNA nanolever (cholesterol intercalates into lipid bilayer). Use low flow rates...
