Restart the device. If this does not help, contact our customer support. As a general note, you should always insert the sample tray and the chip tray completely, i.e. do not instantly stop once you encounter a slight resistance
Thoroughly wash the cell suspension before the measurement and resuspend it by pipetting up and down or pass it through a strainer/filter. In order to avoid aggregation, you can also dilute the cell suspension. Use buffer without Ca2+ and Mg2+ such as our RB1 and/or...
Ensure that your cell suspension is monodisperse (no clumping) and has a concentration of 1 x 106 cells/mL or higher. Resuspend the cell suspension freshly before inserting the samples into the heliXcyto biosensor. You can filter the cell suspension through a cell...
Consider the measurement time in relation to the koff rate. Maybe you are working with a very strong binder and need to extend the dissociation phase. A technical reason might be the capture of autofluorescent dirt particles (often visible in the snapshots). Make sure...
There are several ways to avoid a drift of the signal. First, lower the LED excitation power to below 0.5. If the drift is already visible in the baseline, you can perform a Cyto chip test which includes a bleaching step. If the signal still drifts, you should...
