The Dynamic Response (DR) is a parameter that characterizes the switching speed of DNA nanolevers. It is automatically calculated from the raw data by the switchSENSE® software package. For every recorded data point, the software evaluates typically the upward motion...
The hydrodynamic diameter (Dh) of a molecule is defined as the diameter of a perfect solid sphere that would exhibit the same hydrodynamic friction as the molecule of interest. Thus, the Dh value reflects primarily the hydrodynamic friction but is usually also a good...
The Lollipop model is a biophysical model used in DRX instruments to provide an estimated size of the protein of interest in form of the hydrodynamic diameter. The model assumes that the DNA nanolevers are a charged and rigid cylinder and the attached protein...
Please see section “What are the signals that can be derived from switchSENSE® experiments?”.
Molecular Dynamics Hydrodynamic changes can be detected as changes in the Dynamic Response. Generally, events that increase the hydrodynamic friction, such as binding of an analyte molecule to the ligand, result in a slower switching motion, which can be observed as a...
