Biochips

switchSENSE® Biochips for smart biophysical analysis

 

 

BIOCHIP
SELECTION WIZARD

General Chip Information

Our Biochips

One platform – exceptional information content

DNA-Encoded Addressing

switchSENSE® allows for the parallel functionalization of all electrodes within a single flow channel by hybridization of specifically designed sequences with negligible cross-reactivity. Encode up to six different capture molecules with dedicated complementary nanolever sequences using standard kits, and load them onto the chip in a single step.

Straightforward Functionalization

Ligands are immobilized easily onto a switchSENSE® biochip: Standard coupling kits with, for instance, amino- or thiol-reactive linkers are available for the preparation of DNA-ligand conjugates, allowing for repeated functionalization of the chip via automated DNA hybridization.

Less is More

When it comes to sensor performance, a low number of capture molecules means higher sensitivity and better control over binding stoichiometry. switchSENSE® features microelectrodes which are only 120 μm in diameter, and enables the user to adjust the distance between capture molecules. Through a proprietary desorption method, the density of molecules on the detection spot can be tuned for optimal assay conditions.

Easy Regeneration

The switchSENSE® biochip is functionalized by DNA–DNA hybridization. While being a reliable method to “zip” capture molecules onto the chip, they can be removed as easily by splitting the DNA duplex using an automated routine.
No need to establish unknown regeneration conditions, no need to worry about the activity of capture molecules.

Biochip Design

The switchSENSE® biochip provides 4 flow channels, each with 6 microelectrodes in series. One switchSENSE® sensor spot (≈0.01 mm2) is populated with nanolevers either of one or two populations at a density of around one million nanolevers per spot.

Experimental Cycle

switchSENSE® experiment is straight forward and easy to perform. First you select a biochip according to the objectives of the experiment, second you functionalize the nanolever with a standard coupling kit and third you start the measurement.

The switchSENSE® platform uses one consistent regeneration procedure, irrespective of the type of molecule under investigation. Hence, there is no need to develop or to optimize regeneration conditions.

Used capture molecules are efficiently removed by an automated washing step with a basic solution. While this treatment denatures the base-pairing between tethered and complementary nanolever, the covalently tethered strand remains on the surface for repeated use. Under optimal conditions a nanolever layer can be regenerated more than 20 times with minimal signal loss.

List of switchSENSE® compatible Materials and Conditions

   
BuffersPBS, TRIS, HEPES, MOPS, MES,…pH 5 – pH 10
Salt concentrationMeasurement modes involving electrical actuation
fluorescence measurement modes
1 – 300 mM
0 – 3 M
TemperatureSteady state or temperature gradients up to 50°C/min 8 – 75°C
GlycerolStabilizer and viscous co-solvent0 – 70 wt%
EDTAScavenger for metal ions0 – 5 mM
DMSODissolves polar and non-polar compounds 0 – 5 % v/v
Tween® 20Non-ionic detergent/surfactant (0.05% typical)0 – 1 % v/v
Triton® X-100Non-ionic detergent/surfactant0 – 1 % v/v
Nonidet® P40Non-ionic detergent/surfactant0 – 1 % v/v
Tertigol® NP-40Non-ionic detergent/surfactant0 – 1 % v/v
DDMNon-ionic detergent for membrane proteins
(CMC ~ 0.15 mM)
0 – 20 mM
LipidsSphingolipids, phospholipids (e.g. sphingosine, POPC)0 – 100x CMC
UreaDenaturation agent (non-ionic)0 – 6 M
Guanidine ChlorideDenaturation agent (ionic)0 – 6 M
TCEPReducing agent0 – 1 mM
DTT, MCEReducing agents, use negative electrode potentials<10 µM when switching mM concentrations at negative DC potentials
Serum AlbuminHuman or bovine serum albumin (up to 0.5 mM)0 – serum conc.
SerumHuman serum0 – 50% (1:1)
Cell LysatesLysates, supernatants, …

Please download the switchSENSE® compatibility sheet for more information.

HAVE A QUESTION?

Contact us, we will get in touch with you shortly!

Selection of Biochip

Please follow the 3-step guide below to choose a proper biochip for your experiment.

Step 1 I Type of Biochip

switchSENSE® experiments are performed on a multi-electrode biochip. The biochip features 20 microelectrodes, arranged in 4 separate flow channels that are integrated on-chip for maintenance-free operation. The flow channels are integrated on-chip for maintenance-free operation.

The microelectrode surfaces are functionalized with electrically switchable DNA nanolevers and are ready-to-use. We provide a variety of biochips suitable for different experiments. Please choose from the choices given on the right.

SELECT YOUR TYPE OF BIOCHIP:

Multi-purpose Biochips (MPC) for Binding Kinetics I Binding Affinity I Protein Diameter I Conformational Change I Melting & Thermodynamics I Multimers & Aggregation

Enzymatic Biochips for Nuclease & Polymerase Activity

Bifunctional Biochips for Bispecific Binders & Avidity

Step 2 I Length of Nanolever

We offer the multi-purpose-chip (MPC) in two different nanolever lengths: 48 base pairs (16 nm) and 96 base pairs (32 nm). For target complexes below 100 kDa we recommend the MPC with 48 basepairs: MPC-48. For larger complexes (>100 kDa) we recommend the MPC-96 chip with 96 base pairs. On both types the 5′-end is attached to the surface and the 3′-end carries the fluorophore.

All our biochips come with complementary DNA solution for chip storage.

48 bases (16 nm)

MPC-48-

96 bases (32 nm)

MPC-96-

Step 2 I Length of Nanolever

Use the ENZ-80 biochip to characterize DNA binding enzymes, which need a free 3’-end e.g. polymerases. For improved signal to noise ratio and high-end experiments, we replaced the ENZ-54 biochip with the ENZ-80 biochip.
Please note: On these biochips the 3’-end is attached to the surface & 5’-end carries the fluorophore.

The biochip comes with complementary DNA CNL-P80T80 solution for chip storage. In addition, you receive a 48mer primer to immediately start your polymerase experiments. To resolve fast elongation, we also provide a 36mer primer.

ENZ-80-

Step 2 I Length of Nanolever

For bispecific binders we offer two different ligand distances on one DNA strand: 7 nm and 14 nm; two (different) ligands can be separated 7 nm or 14 nm depending on the structure of the analyte.

All our biochips come with complementary DNA solution for chip storage.

BIF-96-

BIF-96-

Step 3 I Layout of Biochip

The number of different DNA-tether sequences can be chosen according to the experimental requirements. switchSENSE® sensor spots (≈0.01 mm2 ) are populated with nanolevers at a density of around one million nanolevers per spot.

DRX2

DRXred-1

6 single sequence (B) spots (referencing by consecutive experiments)

With 6 single sequence spots, the electrode functionalization features replicates in favor of better measurement statistics.

MPC-48-1-R1-S

MPC-96-1-R1-S

DRXred-2

2 reference (A) & 4 sample (B) spots (end-point referencing in same channel)

The biochip design presenting 2 reference sequence and 4 sample sequence spots enables to measure the reference in the same flow channel.

MPC-48-2-R1-S

MPC-96-2-R1-S

DRX2-2

6 double (A,B) spots (real-time on-spot referencing or sophisticated setups)

On DRX2 biochips, a mixture of two nanolever populations (red & green fluorophors) reside on the same sensor spot. Use one nanolever as the target and the other as the control for absolute data confidence. Of course one can use this set-up also to investigate more complex and challenging issues (e.g. interlinking assays, bispecifics: affinity/avidity,…).

MPC2-48-2-G1R1-S

MPC2-96-2-G1R1-S

DRXred

DRXred-1

6 single sequence (B) spots (referencing by consecutive experiments)

With 6 single sequence spots, the electrode functionalization features replicates in favor of better measurement statistics.

MPC-48-1-R1-S

MPC-96-1-R1-S

DRXred-2

2 reference (A) & 4 sample (B) spots (end-point referencing in same channel)

The biochip design presenting 2 reference sequence and 4 sample sequence spots enables to measure the reference in the same flow channel.

MPC-48-2-R1-S

MPC-96-2-R1-S

DRXyellow

DRXyellow-1

6 single sequence (B) spots (referencing by consecutive experiments)

With 6 single sequence spots, the electrode functionalization features replicates in favor of better measurement statistics.

MPC-48-1-Y1-S

MPC-96-1-Y1-S

DRXyellow-2

2 reference (A) & 4 sample (B) spots (end-point referencing in same channel)

The biochip design presenting 2 reference sequence and 4 sample sequence spots enables to measure the reference in the same flow channel.

MPC-48-2-Y1-S

MPC-96-2-Y1-S

Step 3 I Layout of Biochip

The number of flow channels on the chip as well as the number of different DNA-tether sequences can be chosen according to the experimental requirements. If only few interactions need to be investigated, the electrode functionalization can feature replicates in favor of better measurement statistics. If a large number of diverse interactions are to be investigated simultaneously, the chip can be configured for maximal parallelization.

DRX2

6 single sequence (T) spots (referencing by consecutive experiments)

With 6 single sequence spots, the electrode functionalization features replicates in favor of better measurement statistics.

ENZ-54-1-R1-S

ENZ-80-1-R1-S

DRXred

6 single sequence (T) spots (referencing by consecutive experiments)

With 6 single sequence spots, the electrode functionalization features replicates in favor of better measurement statistics.

ENZ-80-1-R1-S

DRXyellow

6 single sequence (T) spots (referencing by consecutive experiments)

With 6 single sequence spots, the electrode functionalization features replicates in favor of better measurement statistics.

ENZ-80-1-Y1-S

Step 3 I Layout of Biochip

The number of flow channels on the chip as well as the number of different DNA-tether sequences can be chosen according to the experimental requirements. If only few interactions need to be investigated, the electrode functionalization can feature replicates in favor of better measurement statistics. If a large number of diverse interactions are to be investigated simultaneously, the chip can be configured for maximal parallelization.

DRX2

DRXred-2

2 reference (A) & 4 sample (B) spots (end-point referencing in same channel)

The biochip design presenting 2 reference sequence and 4 sample sequence spots enables to measure the reference in the same flow channel.

BIF-96-2-R1-S

DRX2-2-300x48

6 double (A,B) spots (real-time on-spot referencing or sophisticated setups)

On DRX2 biochips, a mixture of two nanolever populations (red & green fluorophors) reside on the same sensor spot. Use one nanolever as the target and the other as the control for absolute data confidence. Of course one can use this set-up also to investigate more complex and challenging issues (e.g. interlinking assays, bispecifics: affinity/avidity,…).

BIF2-96-2-G1R1-S

DRXred

2 reference sequence (A) & 4 probe sequence (B) spots

DRXred-2

2 reference (A) & 4 sample (B) spots (end-point referencing in same channel)

The biochip design presenting 2 reference sequence and 4 sample sequence spots enables to measure the reference in the same flow channel.

BIF-96-2-R1-S

DRXyellow

2 reference sequence (A) & 4 probe sequence (B) spots

DRXyellow-2

2 reference (A) & 4 sample (B) spots (end-point referencing in same channel)

The biochip design presenting 2 reference sequence and 4 sample sequence spots enables to measure the reference in the same flow channel.

BIF-96-2-Y1-S

Standard Grade

Standard grade chips are batch tested, which means selected chips undergo comprehensive quality control (switching dynamics, voltage-response of the DNA layer, and fluorescence levels). In addition, the fluorescence levels on each electrode are tested to be within specifications. Due to the production process not all detection spots fulfill our high quality standards. It is guaranteed that  20 or more detection spots are QC approved. 
We recommend standard grade biochips for testing new assays and to obtain first results.

SELECTED CHIP TYPE MPC-48-1-R1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE MPC-96-1-R1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE MPC-48-1-Y1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE MPC-96-1-Y1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE MPC-48-2-R1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE MPC-96-2-R1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE MPC-48-2-Y1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE MPC-96-2-Y1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE MPC-48-2-G1R1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE MPC2-96-2-G1R1-S
DOWNLOAD CHIP INFORMATION (PDF)

Performance verified chips for use in QC and GxP environments
All biochips are available as performance verified versions upon request. Performance verified chips are especially selected and each detection spot is extensively tested under application conditions and QC approved. Comprehensive QC documentation of performance indicators is provided with every chip for consistent traceability of results.
Recommended for use in QC or GxP environments.

Please contact order@dynamic-biosensors.com for details.

Standard Grade

Standard grade chips are tested for proper fluorescent levels on each spot. Further these chips get batch tested for proper switching dynamics, calibration (voltage-response of the DNA layer) and fluorescence levels hybridization. In this process also each of the four flow channels is regenerated and the hybridization kinetics and efficiencies are recorded in a standardized environment (temperature, buffers, DNA concentration).

SELECTED CHIP TYPE ENZ-80-1-R1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE ENZ-80-1-Y1-S
DOWNLOAD CHIP INFORMATION (PDF)

Performance verified chips for use in QC and GxP environments
All biochips are available as performance verified versions upon request. Performance verified chips are especially selected and each detection spot is extensively tested under application conditions and QC approved. Comprehensive QC documentation of performance indicators is provided with every chip for consistent traceability of results.
Recommended for use in QC or GxP environments.

Please contact order@dynamic-biosensors.com for details.

Standard Grade

Standard grade chips are tested for proper fluorescent levels on each spot. Further these chips get batch tested for proper switching dynamics, calibration (voltage-response of the DNA layer) and fluorescence levels hybridization. In this process also each of the four flow channels is regenerated and the hybridization kinetics and efficiencies are recorded in a standardized environment (temperature, buffers, DNA concentration).

SELECTED CHIP TYPE BIF-96-2-R1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE BIF-96-2-Y1-S
DOWNLOAD CHIP INFORMATION (PDF)

SELECTED CHIP TYPE BIF2-96-2-G1R1-S
DOWNLOAD CHIP INFORMATION (PDF)

Performance verified chips for use in QC and GxP environments
All biochips are available as performance verified versions upon request. Performance verified chips are especially selected and each detection spot is extensively tested under application conditions and QC approved. Comprehensive QC documentation of performance indicators is provided with every chip for consistent traceability of results.
Recommended for use in QC or GxP environments.

Please contact order@dynamic-biosensors.com for details.

Nomenclature of Biochips

The nomenclature of our biochips complies with the following structure:

 

HAVE A QUESTION?

Contact us, we will get in touch with you shortly!

Overview of Biochips

Multi-purpose Chips

Multi-purpose chips are versatile means to analyze protein-protein, protein-small molecule, DNA/RNA-protein, and DNA/RNA-small molecule interactions. By exchanging the standard complementary DNA strand, the chip design enables users to build a near infinite range of molecular structures. Choose from two different nanolevers: standard DNA levers comprise 48 bp and are 16 nm long, or use 96 bp (32 nm) levers for large analytes. Email order@dynamic-biosensors.com for ordering and product information.  

Order No.Lever lengthSequence(s)Fluorescent tagInstrument
MPC-48-1-R1-S486 single sequence spots (B)RedDRXred
DRX2
MPC-48-1-Y1-S486 single sequence spots (B)YellowDRXyellow
MPC-96-1-R1-S966 single sequence spots (B)RedDRXred
DRX2
MPC-96-1-Y1-S966 single sequence spots (B)YellowDRXyellow
MPC-48-2-R1-S48 2 reference sequence spots (A)
and 4 probe sequence spots (B)
RedDRXred DRX2
MPC-48-2-Y1-S482 reference sequence spots (A)
and 4 probe sequence spots (B)
YellowDRXyellow
MPC-96-2-R1-S962 reference sequence spots (A)
and 4 probe sequence spots (B)
RedDRXred
DRX2
MPC-96-2-Y1-S962 reference sequence spots (A)
and 4 probe sequence spots (B)
YellowDRXyellow
MPC2-48-2-G1R1-S486 double-sequence spots (A,B) for on-spot referencing or double probe measurementsGreen and RedDRXred DRX2
MPC2-96-2-G1R1-S966 double-sequence spots (A,B) for on-spot referencing or double probe measurementsGreen and RedDRXred
DRX2

MP3 Chip

The switchSENSE® multi-purpose chip MP3 with its three predefined ligand surface densities makes it easy to experiment with different and ultra-low densities, and ensures quick experimental success even for challenging interactants. For further, detailed information CLICK HERE. Email order@dynamic-biosensors.com for ordering and product information.    

Order No.Lever lengthSequence(s)Fluorescent tagInstrument
MP3-96-2-G1R1-S966 double-sequence spots (A,B) for on-spot referencing or double probe measurementsGreen and Red DRXred DRX2

Bi-functional Chips

Traditional methods used in the study of bi-functional antibodies can often fail to discriminate between complex binding modes (monovalent versus bivalent or bispecific). The new switchSENSE® Bi-functional Chips offer a very flexible and powerful platform for both research and quality control in production. Multiple binding events can be studied either through the use of bi-functional nanolevers that present two different ligands at the same time or through a heterogeneous design where two nanolevers species are immobilized in direct proximity. Email order@dynamic-biosensors.com for ordering and product information.  

Order No.Lever lengthSequence(s)Fluorescent tagInstrument
BIF-96-2-R1-S962 reference sequence spots (A) and
4 probe sequence spots (B)
RedDRXred DRX2
BIF-96-2-Y1-S962 reference sequence spots (A) and
4 probe sequence spots (B)
YellowDRXyellow
BIF2-96-2-G1R1-S966 double-sequence spots (A,B) for on-spot
referencing or double probe measurements
Green and RedDRXred DRX2

 

Enzyme Chips

Our unique enzyme chips are designed to analyze the activity of polymerases and nucleases. Elongation rates, exo- or endonuclease activity, hot start properties, and many other parameters can be analyzed cost efficient and non-hazardous in fully automated, radiation free, and rapid protocols. Label-free polymerase assays are ready-to-use. The nanolever structure consists of a double stranded primer part and a single stranded template. Two versions with different Primer lengths (36 bp Primer and 48 bp Primer) are available: P36T80 and P48T80. The kit includes unlabeled complementary DNA. Email order@dynamic-biosensors.com for ordering and product information.  

Order No.Lever lengthSequence(s)Fluorescent tagInstrument
ENZ-80-1-R1-S806 single sequence spots (B)RedDRXred
DRX2
ENZ-80-1-Y1-S806 single sequence spots (B)YellowDRXyellow

 

Cleaning Chip

Order No.Product description
G-01-1Chip for cleaning purposes with microfluidics, without DNA

Please download the switchSENSE® Biochip Storage Information for optimal storage of your chips. 

Performance verified Chips

Performance verified chips for use in QC and GxP environments
All biochips are available as performance verified versions upon request. Performance verified chips are especially selected and each detection spot is extensively tested under application conditions and QC approved. Comprehensive QC documentation of performance indicators is provided with every chip for consistent traceability of results.

Recommended for use in QC or GxP environments.

Please contact order@dynamic-biosensors.com for details.

Custom-made Chips

Custom biochips with tailor-made surface modifications are available upon request. Please contact order@dynamic-biosensors.com and describe your application. A chip specialist will get in touch with you shortly to discuss technical requirements, solutions, and provide you with timeline and cost estimates.

Didn´t find the information you were looking for?

Contact us, we will get in touch with you shortly!

CONTACT US!
WE WILL GET IN TOUCH WITH YOU SHORTLY.

What are you interested in? *

Consumables & Reagents
Technical Support
Quotes & Orders

Instruments & Applications
Technical & Software Support
Assay Development

New to switchSENSE®
General Info & Evaluation
Purchasing

Dynamic Biosensors Data Privacy Policy

Contact Specialist